摘要目的 观察氟铝联合中毒大鼠血清、骨、牙、脑、肾、肝中的氟、铝含量,探讨氟、铝对上述组织的影响机制.方法 采用成组设计,选取4周龄SPF级Wistar大鼠40只,雌性24只、雄性16只,体重40～ 50g,按照体重采用随机数字表法分为对照组、加氟组、加铝组和砖茶组(每组10只大鼠,雌性6只、雄性4只),对照组饮用日常饮用水;加氟组饮用氟含量为100 mg/L的溶液;加铝组饮用铝含量为159 mg/L的溶液;砖茶组依据牧民的饮茶习惯和饮茶量计算出大鼠的饮茶浓度及饮茶量,按此比例熬出茶汤,其氟含量为(100±2)mg/L,铝含量为(159.0±1.5)mg/L.干预1年后处死大鼠,测定血清、骨、牙、脑、肾、肝的氟、铝含量.结果 砖茶组大鼠骨、牙、肝氟含量[(275.81±55.89)、(283.32±70.67)、(15.52±7.57)μg/g]显著高于对照组[(0.04±0.01)、(128.52±12.81)、(5.89±0.33)μg/g,P均＜0.01];砖茶组大鼠牙和肝铝含量[(6.14±1.69)、(8.97±0.85)μg/g]显著高于对照组[(0.72±0.54)、(5.47±0.83) μg/g,P均＜0.01].结论 砖茶型氟铝联合中毒能引起骨、牙、肝氟含量增加,牙、肝铝含量增加.砖茶型氟铝联合中毒大鼠损伤的主要组织是骨、牙齿以及肝脏.

abstractsObjective To observe the effect of fluorine (F) and aluminum (Al) on serum,bone,teeth,brain,kidney and liver tissues,contents of F and Al in different tissues of rats with combined F-Al toxicosis caused by drinking brick tea were determined.Methods Forty SPF grade Wistar rats of 4 weeks,including 24 females and 16 males,weighing between 40 and 50 g,were divided into control group,F group,Al group and brick tea group by random number table according to their weight,ten rats (6 females,4 males) in each group.The control group received daily drinking water.The F group drank a solution with an F content of 100 mg/L,and the Al group drank a solution with an Al content of 159 mg/L.According to the drinking habits and consumption of the herdsmen,the tea concentration and tea consumption of the rats were calculated for the brick tea group,and a tea soup was made according to the ratio,with the F content of (100 ± 2) mg/L and the Al content of (159.0 ± 1.5) mg/L.One year later,the rats were killed to determine the contents of F and Al in serum,bone,teeth,brain,kidney and liver.Results The contents of F in bone,teeth and liver of the brick tea group [(275.81 + 55.89),(283.32 ± 70.67),(15.52 ±7.57) μg/g] were significantly higher than those of the control group [(0.04 ± 0.01),(128.52 ± 12.81),(5.89 ±0.33) μg/g,P ＜ 0.01].The Al contents of teeth and liver in the brick tea group [(6.14 ± 1.69),(8.97 ± 0.85) μg/g]were significantly higher than those of the control group [(0.72 + 0.54),(5.47 + 0.83) μg/g,P ＜ 0.01].Conclusions F-Al combined toxicosis caused by drinking brick tea can cause the increases of F contents in bone,teeth and liver,and the increases of Al contents in teeth and liver.The main tissues damaged by F-Al combined toxicosis caused by drinking brick tea in rats are bone,teeth and liver.