摘要目的 研究荧光定量聚合酶链反应(PCR)技术检测临床标本中结核分枝杆菌核酸DNA的应用价值.方法 应用荧光定量PCR技术对80例活动性肺结核患者痰、40例结核性胸膜炎患者的胸水进行结核杆菌DNA检测,同时涂片镜检与培养,并对3种方法检测的阳性率进行比较.结果 80例活动性肺结核患者痰、40例结核性胸膜炎患者的胸水荧光定量PCR阳性率分别为56.2％、27.5％；涂片镜检阳性率分别为16.2％、0.0％；结核培养法阳性率分别为37.5％、2.5％.结论 荧光定量PCR技术较传统方法具有较高的敏感性与特异性,尤其对涂片染色与结核培养阴性的结核病具有更大的诊断价值.

abstractsObjective To value fluorescent quantified PCR (polymerase chain reaction) technique to detect the DNA of Mycobacterium tuberculosis from clinical patients.Methods Detect the DNA of TB from the sputum of 80 active tuberculosis and hydrothorax of 40 tuberculous pleuritis.At equal pace,make a comparison to proceed microscopic examination and cultivation.Results With fluorescent quantified PCR,56.2％ (45/80),27.5％ (11/40)positive detection from active tuberculosis and tuberculous pleuritis respectively.16.2％ ( 13/80 ),0.0％ positive specimen were detected with microscopic examination in two group of patients.Cultivation got 37.5％ (30/80),2.5％(1/40) positive detection respectively.Conclusion Fluorescent quantified PCR was more sensitive and specific than traditional methods,and valuable in the microscopic examination and cultivation negative patients especially.