摘要目的 探讨司坦唑醇(stanozolol,ST)对离体培养的促性腺激素释放激素拟似物(gonad0-tropin releasing hormone agonist,GnRHa)处理后青春期大鼠生长板软骨细胞的Ⅱ、X型胶原合成的影响.方法 将6只经GnRHa处理后的雌鼠原代软骨细胞分为时效组(观察ST干预时限的影响)、量效组(观察ST干预剂量的影响).采用免疫组化法检测ST对软骨细胞Ⅱ、X型胶原的表达.结果 ST作用3 d后胶原Ⅱ的表达明显高于对照组(P<0.05),但至第5天却表达下降;而3 d内胶原X分泌量与对照组比较无差异,4天起显著递增(P=0.000).ST各个浓度的软骨细胞胶原Ⅱ表达较基值均明显增加(P<0.01),并以10-9最显;10-11 M～10-8 M的ST不增加胶原X表达,至10-7 M始随剂量增加而增,10-5 M时达最高值(P<0.01).结论 ST对Ⅱ、X胶原的量效和时效影响存在"错峰"改变,时效影响的差异较量效差异为显著.对保持细胞生长/成熟正平衡,时效影响更需关注.

abstractsObjective To observe the effects of stanozolol on collagen I1 and X synthesization of cul- tured growth plate ehondrocytes from pubertal female rat treated with GnRHa in vitro. Methods Primary Chon-drocytes from 6 female rats were divided into two groups ( time-effect groups and concentration-effect groups). immunohistochemical staining of collagen Ⅱ, X were conducted. Results the results of type Ⅱ collagen synthe- sization demonstrated stanozolol enhanced the proliferation of the chondrocytes, time-course studies had shown that the proliferation were maximally stimulated by stanozolol after 3 days of incubation and decreased again after longer periods of incubation. Stanozolol did not stimulated type X collagen synthesization from 10-11 M-10-8 M, but experiments showed that type X collagen was already stimulated after incubation in stanozolol (10-7-10-5 M). Time-course studies had shown those type X collagen synthesizations were stimulated by stanozolol after 4-5 days of incubation. Conclusion Stanozoiol enhances the proliferation of ehondrocytes of pubertal female rat treated with GnRHa in vitro( time-course-dependent and concentration-dependent).