摘要目的 探讨支气管哮喘(哮喘)患者呼气冷凝液(EBC)中氮氧化物与气道炎症的关系.方法 哮喘患者急性发作期30例,缓解期13例,健康对照组30例,收集其EBC,用分光光度法检测EBC中NO2-、NO3-、总NO2-/NO3-浓度.结果 哮喘急性发作期EBC中NO2(1.508±0.705)μmol/L显著高于健康对照组(0.605±0.299)μmol/L,(P＜0.01);缓解期NO2-(0.622±0.253)μmol/L较急性发作期显著下降(P＜0.01).EBC中NO3-、总NO2-/NO3-的浓度在急性发作期及缓解期变化均无显著性差异(P＞0.05).结论 EBC中NO2-水平高低与哮喘病情变化有关,故可作为哮喘患者气道炎症反应的一项重要监测指标.

abstractsObjective To investigate the relationship between nitrogen oxides of exhaled breath condensate(EBC) and the airway inflammation in patients with asthma.Methods Thirty patients with asthma exacerbation,13asthma remission and 30 healthy controls performed EBC collection.NO2-,NO3- and total NO2-/NO3- in EBC were measured by the spectrophotometry.Results The level NO2-(1.508±0.705)μmol/L in EBC of asthma exacerbation group was significantly increased than that in control group(0.605±0.299)μmol/L (P＜0.01).NO2- in EBC showed decreased significantly in remission groud than that in exacerbation groud (P＜0.01).The levels of NO3- and total NO2-/NO3- were no significant difference in all groups.Conclusion These findings suggest that inflammation was characteristic in the airways of asthma patients.The levels of NO2 in EBC are associated with the change of asthma and may prove to be useful inmonitoring of airway inflammation in asthma.