摘要目的 观察丙型肝炎病毒NS3蛋白对QSG7701细胞survivin的影响及其与细胞凋亡的关系,以探讨HCV NS3蛋白是否通过调节survivin的表达来参与对细胞凋亡的调控,进而参与HCV相关性肝癌的发生.方法 采用Western blot、免疫细胞化学检测血清饥饿前后细胞survivin蛋白表达的变化,采用TUNEL法检测血清饥饿前后细胞凋亡的变化.结果 HCV NS3蛋白促进QSG7701细胞survivin的表达,血清饥饿诱导凋亡后,survivin表达降低,HCV NS3蛋白亦表现出促进survivin表达的作用,效果呈浓度依赖性和时间依赖性.经血清饥饿诱导凋亡后,HCV NS3蛋白抑制凋亡.结论 HCV NS3蛋白可能通过促进QSG7701细胞survivin的表达从而抑制细胞凋亡,对HCV相关性肝癌的形成有一定作用.

abstractsObjective To study the regulation of HCV NS3 protein on the expression of survivin prorein in QSG7701 cell and the interacion with apoptosis for investigating carcinogenesis mechanism of HCV NS3 protein in human liver cell. Methods Apoptosis induced by serum starvation or not, the expressions of survivin protein are detected by Western Blot and immunocytochemistry, the apoptosis indexes are examined by TUNEL.Results HCV NS3 protein activates the expression of survivin in QSG7701 cell. After apoptosis induced by serum starvation, the expressions of survivin are down-regulated. HCV NS3 protein also activates the expression of survivin which exhibits the tendency of time and concentration in QSG7701 cell. At the same time, HCV NS3 protein inhibits apoptosis induced by serum deprivation in QSG7701 cell. Conclusion HCV NS3 protein inhibits apoptosis by up-regulating the expression of survivin protein in QSG7701 cell, contributes to malignant transformation of hepatocytes and hepatocellular carcinogenesis related HCV.