摘要目的 探讨自体血清替代胎牛血清培养经血源性基质干细胞的可行性,从而为疾病的个体化治疗提供参考.方法 收集20~30岁健康育龄女性的月经血,通过密度梯度离心法分离出经血源性基质干细胞,分别均匀地接种于含10%胎牛血清的DMEM/F-12培养体系和含10%自体血清的DMEM/F-12培养体系中,观察细胞的形态及增殖特性,并采用CCK-8法绘制生长曲线.比较两种培养体系培养的经血源性基质干细胞的成骨及成脂能力.结果 两种培养体系下,经血源性基质干细胞均呈典型的短小梭形生长,均可形成集落.成人自体血清组培养的经血源性基质干细胞在增殖速度上接近于胎牛血清组,CCK-8生长曲线的吸光度值差异无统计学意义(P>0.05).结论 两种培养体系下的经血源性基质干细胞均具有成骨及成脂能力.成人自体血清组培养的经血源性基质干细胞的形态和增殖能力接近于胎牛血清组,并维持了经血源性基质干细胞的基本生物学特性,可以替代胎牛血清用于经血源性基质干细胞的扩增培养,作为一种无异种血清的培养体系,为大量扩增经血源性基质干细胞用于开展临床治疗提供了较为安全的选择.

abstractsObjective To investigate the feasibility of culturing menstrual blood mesenchymal stem cells in autologous serum system and provide individualized treatment.Methods Collecting the menstrual blood of healthy and child-bearing women of 20-30 years old.Isolated the menstrual blood mesenchymal stem cells through ficoll-hypaque density gradient centrifugation,and suspended them separately,even in DMEM-F12 medium which supplemented with 10% fetal bovine serum and with 10% autologous serum.Supervise the morphology and proliferation of the cultured cell,and draw the growth curves with CCK-8 assay.Compare their osteogenic and adipogenic potential.Results The menstrual blood mesenchymal stem cells cultured in the two systems both showed to be typical spindle shaped and can form colony,and their proliferation ability was similar,with CCK-8 assay,the absorbance value of both system had no significant difference(P>0.05).Conclusion Both of the training systems have the osteogenic and adipogenic potential.The menstrual blood mesenchymal stem cells are cultured in the two systems both have typical spindle shape and colonic growth,and their proliferation abilities are similar,maintaining the menstrual blood of basic biological characteristics,which can replace fetal bovine serum stromal stem cells being used in menstrual blood amplification culture,as the same kind of serum cultivation system,for a large number of amplification menstrual blood source sex stromal stem cells being used in clinical treatment and provides a relatively safe choice.