换一批
换一批摘要Objective To investigate the combination of Epstein-Barr virus nuclear protein 3C (EBNA3C) with Gemin3 and its effect on Gemin3 expression.Methods Co-immunoprecipitation,GST pull-down and immunofluorescent assay were used to determine the combination of EBNA3C and Gemin3 and their combining domain.Stable EBNA3C knockdown cell lines were made by lentivirus-delivered small hairpin RNA and then puromycin selection.Western blot was used to check the effect of EBNA3C on Gemin3 expression.Results EBNA3C and Gemin3 combined with each other in vivo and in vitro through their C-terminals.EBNA3C up-regulated Gemin3 gene expression.Conclusion EBNA3C forms complex with Gemin3 and up-regulates its expression.
更多相关知识
- 浏览115
- 被引0
- 下载4
相似文献
- 中文期刊
- 外文期刊
- 学位论文
- 会议论文
