摘要Objective To investigate the effect of sevoflurane preconditioning and postconditioning on lung ischemia-reperfusion (IR) injury and apoptosis in rat.Methods Wistar rats were randomly assigned to four groups:sham group (n =6):no ischaemia-reperfusion; IR group (n =6):left lung ischemia was achieved by clamping the hilum for 90 min,followed by 120 min reperfusion; sev+pre group (n =6):1 minimum alveolar concentration (MAC) sevoflurane was administered for 30 min prior to ischemia; sev+post group (n =6):ischemia was followed by 1 MAC sevoflurane postconditioning at the first 30 min reperfusion.PaO2 was measured after reperfusion.The number of apoptotic cells was estimated using the terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick-end labeling (TUNEL) technique.Results After ischemia-reperfusion,a significant deterioration of PaO2 was noticed and the number of apoptotic cells remarkably increased compared with that of sham group.In sev+pre group and sev+post group,PaO2 was (85.7± 14.4) mmHg and (88.6± 12.5) mmHg respectively,which was apparently increased compared with that in IR group [(63.9± 11.3) mmHg,P ＜0.05].The number of apoptotic cells in sev+pre group [(6.94 ± 1.49)％] and sev+post group [(7.69 ± 1.61)％] was significantly lower than that in IR group [(12.12 ± 2.77)％,P ＜0.05].But all parameters showed no significant difference between sev+pre group and sev+post group.Conclusions Both sevoflurane preconditioning and postconditioning could prevent lung ischemiareperfusion injury and attenuate apoptosis in rat.