摘要目的　检验神经元cdc-2类激酶（Cdk5/p35nck5a）在神经元成熟和萌芽中扮演着重要作用的假说。方法　采用Western blot分析,免疫组织化学染色和免疫沉降Cdk5活性分析的方法研究了在癫痫各阶段大鼠海马区神经元cdc-2类激酶的活性变化、Cdk5和p35nck5a的表达水平及细胞内的分布。结果　在癫痫大鼠脑中的活性比正常大鼠脑中的活性呈有意义的增高，在所有癫痫各阶段的神经元cdc-2类激酶活性中，第三阶段的活性是最高的。该激酶活性的变化与p35nck5a表达的变化是一致的。Cdk5的表达量在癫痫各阶段中是恒定不变的，但是，它在细胞内分布出现了引人注目的变化，当Cdk5活性高时Cdk5的分布从轴索转移到细胞质，p35nck5a的分布一直在细胞质内。结论　神经元cdc-2类激酶在轴索突触再组成上扮演着重要的作用，而且，Cdk5的分布从轴索转移到细胞质的过程可能是控制其活性的一个新调控机理。

abstractsObjective　To test the hypothesis that neuronal cdc2-like kinase (Cdk5/p35nck5a) plays an important role in neuronal maturation and sprouting.Methods　Changes kinase activity, expression levels and subcellular localizations of Cdk5 and p35nck5a in the rat hippocampus were studied during kindling progression by Western blot analysis, immunohistochemitry, immunoprecipitation and kinase assay. Results　Kinase activity in kindling rats was significantly higher than that in normal adult rats. The kinase activity at stage 3 was most prominent among all stages of kindling progression. The changes in kinase activity coincided with those of p35nck5a expression in kindling rats. In contrast, the expression of Cdk5 was constant throughout the progression of kindling stages. However, subcellular localization of Cdk5 dramatically changed in the hippocampal neurons of kindling rats. Cdk5 was translocated from axon to soma when kinase activity was high. p35nck5a was always localized in the soma throughout kindling progression.Conclusions　Neuronal cdc2-like kinase plays an important role in synaptic reorganization, and the translocation of Cdk5 to the soma from the axon may be a novel regulatory mechanism to control kinase activity.