摘要目的　为研究IL-2R基因转染PBL对人卵巢癌细胞生长抑制的影响。方法　用脂质体Fugene作载体将IL-2和IL-2R基因分别转染入人卵巢癌细胞株3AO和人外周血白细胞(PBLs),转染24小时后分别将转染和非转染的PBLs与转染和非转染的3AO细胞混合培养48小时,并用MTT法测定PBLs对3AO细胞的杀伤作用。结果　转染前后3AO细胞和PBLs细胞形态无明显变化。3AO能转染IL-2基因并表达IL-2mRNA,PBLs能转染IL-2R基因并表达IL-2RmRNA。IL-2基因修饰3AO细胞可增强淋巴细胞对3AO细胞的抑制作用,同时联合IL-2R基因转染PBLs和IL-2基因修饰3AO细胞可显著增强其淋巴细胞对3AO细胞的抑制作用。结论　IL-2R基因转染效应细胞可增加IL-2基因转染肿瘤细胞的抗肿瘤免疫效应,这一方法为卵巢癌IL-2基因治疗开辟了新的治疗途径。

abstractsObjective　To investigate the growth inhibition of interleukin-2 receptor (IL-2R) gene-transduced peripheral blood lymphocytes (PBLs) on human ovarian cancer cells.Methods　Interleukin-2 (IL-2) and IL-2R genes were transfected into human ovarian cancer cell line 3AO and PBLs, respectively, using the same Fugene vector. Twenty-four hours later transfected and nontransfected PBLs were cocultured with transfected and nontransfected 3AO for 48 hours. Cytotoxity of PBLs on 3AO was detected by the MTT assay.Results　The morphology of IL-2-transduced 3AO and IL-2R-transduced PBLs remained unchanged. 3AO cells could be transfected with the IL-2 gene and expressed IL-2 mRNA, and PBLs could be transfected with the IL-2R gene and expressed IL-2R mRNA. IL-2 transduced 3AO cells enhanced their response to the cytotoxity of PBLs. Furthermore, growth inhibition of PBLs to 3AO cells increased significantly when the IL-2R was transfected into PBLs and when the IL-2 gene was transfected into 3AO cells and the two were combined.Conclusions　IL-2R gene transduced PBLs are able to enhance their cytotoxity on IL-2 gene transduced ovarian cancer cells. This method may be a new way to investigate IL-2 gene therapy for ovarian cancer.