摘要目的　明确人鼻粘膜上皮细胞Na+通道的特性，为研究Na+通道在鼻粘膜病理性改变及治疗中的作用奠定理论基础。方法　利用膜片钳技术对经无血清气-液界面培养的鼻源性鼾症患者手术切除下鼻甲标本的鼻上皮细胞进行Na+通道基本特性研究。结果　在细胞贴附式膜片上，可记录到典型的单通道电流，其电导为21.09pS，反转电位为-50.96mV，且77.78%反转电位<-40mV，离子通透性比率PNa/PK>5.80。在Na+通道抑制剂10-4 mmol/L Amiloride存在于电极液内时，Na+通道发生率从26.7%减少到5.13%（P<0.05），表明大部分Na+通道可被Amiloride抑制。10-3 mmol/L Ca2+存在于浴液时, Na+通道的发生率无明显增加（P>0.05）。电压对开放概率无明显影响。结论　在细胞贴附式膜片上，人鼻粘膜上皮细胞具有大部分Amiloride敏感型的Na+选择性通道及小部分Amiloride非敏感型的Na+通道。细胞外液的Ca2+不能激活Na+选择性通道的开放，通道的开放概率是非电压依赖性的。

abstractsObjective　To study the electrophysiological properties of sodium channels in the apical membrane of human nasal epithelial cells.Method　Nasal epithelial cells of human inferior turbinate from patients with obstructive sleep apnea syndrome were cultured in serum free medium on collagen gel-coated membranes at an air-liquid interface and studied by a patch clamp technique. Results　In cell-attached patches, a typical single channel current with a conductance of 21.09pS and reversal potential of -50.96 were recorded. The permeability ratio PNa/PK was more than 5.80. In the presence of 10-4 mmol/L amiloride in the pipette, the incidence of sodium channels decreased from 26.67% to 5.13%. This revealed that a population of channels were inhibited by amiloride at a dose of 10-4 mmol/L. Ca2+ at dose of 10-3 mmol/L did not influence the incidence of sodium channels. There was no obvious association between voltage and the open probability of the channels. Conclusions　Our results indicate that most Na+ channels in cell-attached patches of human nasal epithelial cells are amiloride-sensitive and Na+ selective. Only a few channels are amiloride-insensitive. The channels were not activated by extracellular Ca2+ and the open probability followed a voltage-independent manner.