摘要目的探讨脂质体-硫代磷酸化修饰的C-erbB2反义脱氧寡核苷酸(C-erbB2 antisense phosphorothioated oligodeoxynucleotides, C-erbB2 S-ODNs)对卵巢癌细胞系C-erbB2癌基因表达及细胞增殖的影响.方法采用流式细胞仪技术,3H-TdR掺入方法了解脂质体-C-erbB2 S-ODNs对卵巢癌细胞系SKOV3 C-erbB2癌基因表达及细胞生长,细胞周期的作用.结果脂质体-C-erbB2 S-ODNs能降低C-erbB2的表达,并抑制细胞生长达30%;脂质体-C-erbB2 S-ODNs较单用C-erbB2 S-ODNs的作用效率增强约40倍.结论 C-erbB2 癌基因的反义调控可能在卵巢癌基因治疗中有一定作用;脂质体能够有效地增强C-erbB2 S-ODNs的作用效率.

abstractsAbstract:Objective To explore the effects of liposome-C-erbB2 antisense phosphorothioate oligodeoxynucleotides (S-ODNs) on C-erbB2 proto-oncogene expression and cell proliferation in human ovarian cancer cells.Methods The effects of liposome-C-erbB2 S-ODNs on C-erbB2 protein expression, cell cycle and cell proliferation in human ovarian cancer cells were studied by means of flow cytometry and 3H-thymidine incorporation.Results Liposome-C-erbB2 S-ODNs can specifically reduce C-erbB2 protein expression in human ovarian cancer cells, accompanied by a 30% inhibition of cell proliferation. The effectiveness of liposome-C-erbB2 S-ODNs on the expression of C-erbB2 was about 40 times higher than that of C-erbB2 S-ODNs.Conclusions The data suggest that antisense therapy might be a useful method of gene therapy in ovarian cancer. The effectiveness of C-erbB2 S-ODNs could be greatly increased by adsorption of S-ODNs by liposomes.