摘要目的越来越多的资料表明肿瘤是一类细胞周期性疾病,从而使得细胞周期分析在细胞生物学,肿瘤生物学领域显得格外重要.迄今为止,有很多方法应用于细胞周期分析中,但这些方法因为这样或者那样的缺点已无法适应今天的细胞周期分析.本研究的目的是建立一种新的,更为合理的细胞周期分析方法.方法将cyclin E以及cyclin A的单克隆抗体按一定的比例混合后与固定了的MOLT-4细胞孵育,后用流式细胞仪检测.结果我们发明的Cyclin E+A/DNA多参数流式细胞术能将细胞分为六个细胞群体:G0、早G1、晚G1、S、G2、M期细胞.而在DNA含量直方图仅能区分三个细胞群体:G0/G1、S、G2/M期细胞.结论 Cyclin E+A/DNA多参数流式细胞术能同时在同一样本中将细胞分为六个细胞群体:G0、早G1、晚G1、S、G2、M期细胞.其对细胞周期分析明显优于目前采用的细胞周期分析方法,并且有明显的生物学基础.

abstractsObjective　To establish a new method for analyzing the cell cycle in tumor which is a kind of cell cycle disease.Methods　Mixtures of cyclin E and cyclin A antibodies were incubated with fixed MOLT-4 cells, and measured by flow cytometry.Results　We developed a cyclin E+A/DNA flow cytometry analysis method, which may distinguish G0, early G1, late G1, S, G2 and M phase cells, rather than three phases in the DNA content histogram.Conclusion　Cyclin E+A/DNA multiparameter flow cytometry can simultaneously differentiate in the same sample six cell groups: G0, early G1, late G1, S, G2 and M phase cells. It performed better than any other cell cycle analysis methods that we have used and has a definite cell biology foundation.