摘要目的研究电磁脉冲(EMP)对犬淋巴细胞的损伤效应，并探讨其作用机理。方法用酸性磷酸酯酶细胞化学方法检测T、TH和Ts淋巴细胞，用原位末端标记和免疫细胞化学技术分析淋巴细胞凋亡和Bcl-2、Bax蛋白的改变。结果 (2-12)×104 V/m的场强能使外周血T细胞及其亚群出现明显降低；凋亡淋巴细胞随照射剂量的增加而明显升高；不同剂量照射后10天，淋巴细胞Bax蛋白的表达明显增强，与淋巴细胞凋亡呈现出比较好的相关性，而Bcl-2蛋白显著降低。结论首次证实一定场强的EMP确实能损伤淋巴细胞，并呈现出较好的剂量效应关系。淋巴细胞凋亡是EMP引起淋巴细胞死亡和机体免疫功能降低的主要原因，Bax和Bcl-2在上述凋亡调控中起着重要作用。

abstractsObjective To study the effects of electronic magnetic pulse (EMP) on peripheral lymphocytes in dogs and to explore the mechanisms of the biological effects of EMP.Methods T, TH and Ts lymphocytes were estimated by acid phosphatase cytochemistry. Apoptotic lymphocytes and Bax and Bcl-2 proteins related to apoptosis were observed with in situ terminal labeling and immunocytochemistry.Results Peripheral T lymphocyte subpopulations decreased obviously after EMP irradiation with (2 - 12) × 104 V/m. Apoptotic percentages of lymphocytes increased with the elevation of EMP doses. Ten days after different intensity radiation, the Bax protein was found to be elevated in accord with the peak value of lymphocyte apoptosis. However, Bcl-2 protein decreased obviously.Conclusion A definite field intensity EMP could induce injury to lymphocytes. Apoptosis induced by EMP is one of the main causes of peripheral lymphocyte death and leads to immunosuppression of the body.These results suggest that people should pay more attention to the injury caused by EMP, especially to the immunological functions of the body.