摘要目的奥曲肽能抑制人肝癌的生长,其机制尚未明了,本研究旨在观察奥曲肽在体外能否诱导人肝癌细胞凋亡,以从诱导肿瘤细胞凋亡的角度来探讨其抑制人肝癌生长的机制.方法将奥曲肽作用体外培养的人肝癌细胞株,运用细胞化学染色(Hochesst 33 258),透射电子显微镜术,DNA琼脂糖电泳及流式细胞术(flow cytometry,FCM)等方法来检测凋亡.结果经0.2μg/ml的奥曲肽处理后,形态学上,BEL-7402肝癌细胞表现为细胞皱缩,核质浓缩、核碎裂,细胞起泡以及凋亡小体形成等凋亡特征性的形态学改变;生化学上,DNA琼脂糖电泳呈现为梯状带.经FCM定量发现,随着奥曲肽之浓度增高(0～2 μg/ml),凋亡细胞增多,而且凋亡率与奥曲肽的药物浓度呈正相关(r=0.809,P＜0.05).结论在体外,奥曲肽可诱导人肝癌细胞凋亡,这可能为奥曲肽抑制人肝癌生长的机制.

abstractsObjectives To investigate whether apoptosis can be induced by Octreotide in human hepatoma cells in vitro and elucidate the antineoplastic mechanism of Octreotide in hepatoma.Methods A cultured human hepatoma cell line,BEL-7402,was exposed to Octreotide and apoptosis was evaluated by cytochemical staining(Hochesst 33258),transmission electron microscopy,agarose gel electrophoresis and flow cytometry(FCM).Results After exposure to 0.2 μg/ml Octreotide,apoptosis with nuclear chromatin condensation as well as fragmentation,cell shrinkage and the formation of apoptotic bodies was observed using cytochemical staining and transmission electron microscopy.A DNA ladder in agarose gel electrophoresis was also displayed.FCM showed that the apoptotic cell number rose with an increase in the concentration of Octreotide(0- 2 iμg/ml).There was a positive correlation between Octreotide concentration and apoptotic rate in BEL-7402 cells(r=0.809,P＜0.05).Conclusion Apoptosis in human hepatoma cells can be induced by Octreotide,which may be related to the mechanism of antineoplastic action of Octreotide in hepatoma.