摘要目的建立鼠慢性萎缩性胃炎(CAG)动物模型并探讨致萎缩因素。方法应用60%酒精和20mmol/L去氧胆酸钠(A因素)，0.05%-0.1%氨水(B因素)，0.05%吲哚美辛(C因素)按有重复的L8(27)正交设计对SD大鼠分三批进行制模刺激，三批刺激时间分别为3个月，6个月，9个月。实验到期后取出全胃，观察大鼠胃黏膜大体观及各项萎缩指标。结果应用A、B、C三因素(单因素或多因素)刺激6个月或9个月均可出现典型的胃窦萎缩性胃炎征象，且制模成功率达100%，该两批建成的CAG模型其胃黏膜基本病理变化无明显差异，而制模刺激3个月则无明显的胃黏膜萎缩出现。结论应用60%酒精，20mmol/L去氧胆酸钠，0.05%-0.1%氨水，0.05%吲哚美辛对SD大鼠刺激6个月可成功的建立CAG模型，延长刺激时间并不能进一步加重鼠胃黏膜萎缩程度。

abstractsObjective To establish a rat model of chronic atrophic gastritis and explore the factors inducing atrophy.Methods In accordance with repeated orthogonal design of L8(27), 60% alcohol and 20mmol/L sodium deoxycholate (served as factor A), 0.05%-0.1% ammonia water (factor B), 0.05% indomethacin (factor C) were given, alone or in combination, to rats in three experiments for 3 months, 6 months or 9 months respectively. Then the rats were dissected, and their pathologic changes of the gastric mucosa were assessed.Results Typical signs of chronic atrophic gastritis (CAG) were found in all rats which were treated with factor A, B, C alone or in combination for 6 or 9 months. No significant difference of pathologic changes of gastric mucosa was found between the rats treated for 6 months and those for 9 months. No obvious CAG signs were found in the rats treated with factor A, B, C for 3 months.Conclusion Sixty percent of alcohol, 20mmol/L sodium deoxycholate, 0.05%-0.1% ammonia water and 0.05% indomethacin given to Sprague-Dawley rats for 6 months can successfully establish the animal model of CAG. Prolongation of the model-establishment time is not able to further facilitate the atrophy of gastric mucosa.