摘要目的　探讨参麦注射液(SI)对烫伤小鼠腹腔巨噬细胞TNF-α mRNA表达的影响。方法　致BALB/C小鼠占体表面积11% 的Ⅲ度烫伤，每日腹腔注射SI，连续5天。半定量RT-PCR测定腹腔巨噬细胞TNF-α mRNA表达。结果　烫伤小鼠腹腔巨噬细胞TNF-α mRNA 表达显著增高，而SI可使之显著降低（P<0.01）并明显提高烫伤小鼠的存活率（P<0.05）。结论　烫伤小鼠早期死亡原因可能与腹腔巨噬细胞TNF-α mRNA高 表达有关。SI可降低烫伤小鼠腹腔巨噬细胞TNF-α mRNA的高表达，提高烫伤小鼠存活率。

abstractsObjective To explore the effect of shenmai injection (SI) on expression of TNF-α mRNA in peritoneal macrophages (pMΦs) of scald mice.Methods BALB/c mice were inflicted with 11% of body surface area Ⅲ degree scald and injected intraperitoneally (ip) with SI daily for 5 days, and expression of TNF-α mRNA in pMΦs was determined by semi-quantitative RT-PCR.Results In scald mice, the expression of TNF-α mRNA in pMΦs increased significantly, but it was reduced obviously (P<0.01) after SI administration, while the livability was increased markedly (P<0.05).Conclusions For scald mice, the cause of death at early stage might be related to the high expression of TNF-α mRNA in pMΦs and the use of SI can decrease the death rate.