摘要目的　测定血清可溶性幽门螺杆菌抗原(S-Hp)和其特异性免疫复合物(Hp-IC)并评价它们对幽门螺杆菌感染诊断的意义.方法　采用双抗体夹心式酶免疫测定法.结果　66例Hp感染患者血清S-Hp阳性率90.91%,显著高于28例阴性对照组阳性率0%,P<0.001.S-Hp含量与Hp感染菌量呈成比,但43例Hp阳性患者治疗前后S-Hp 含量无显著改变（P>0.05）.血清 S-Hp抗原均以IgG和/或IgA型特异性免疫复合物形式存在,Hp-IC对Hp感染诊断特异性85.71%,敏感性77.23%. 结论　S-Hp和Hp-IC测定可用于临床Hp感染诊断,对阐明Hp致病机理有重要意义.

abstractsObjective To explore the characteristics of Helicobacter pylori (H.pylori) antigen in serum and to evaluate its clinical diagnostic value. Methods Enzyme-linked immunosorbant assay (ELISA) was developed to detect the soluble H.pylori antigen (S-Hp) and circulatory specific H.pylori antigen immunocomplexes (Hp-IC) in serum. Results The positive rate of S-Hp was 90.91% from 66 patients with H. pylori infection, which was much greater than 0% found in 28 controls (P<0.001). Moreover, its concentration closely reflected the number of H.pylori in the gastric mucosa layer. We also found that Hp-IC existed bound with IgG and/or IgA in patients with positive S-Hp. However, there is no evidence to show the concentration of S-Hp reduced significantly in followed-up subjects after effective therapy. Conclusions These methods as newly and noninvasive complementary tools can be used for clinical diagnosis of H.pylori infection. In addition, S-Hp and Hp-IC may be of importance in H.pylori pathogenesis.