摘要目的研究硫酸多糖916(PS916)对TNFa、IL-1β和H2O2诱导ECV304细胞产生一氧化氮(NO)的影响.方法用Griess试剂测定ECV304细胞产生的NO,用MTT法测定细胞的增殖,用荧光法测定NO合酶的活性. 结果 40ng/ml TNFa和40ng/ml IL-1β使ECV304细胞NO产生下降,0.1mmol/L H2O2使NO的产生增加.PS916剂量依赖性增加TNFa、IL-1β诱导的ECV304细胞NO的产生,降低H2O2诱导ECV304细胞产生的NO.TNFa和H2O2抑制ECV304细胞的增殖,而IL-1β对ECV304细胞的增殖没有明显影响.PS916剂量依赖性的抑制TNFa和H2O2的作用,增加存活细胞数.在体外,PS916对细胞的NO合酶无明显的影响.结论 PS916在体外实验中对细胞因子和H2O2引起的ECV304细胞损伤有拮抗作用,对细胞表现出明显的保护作用.

abstractsObjective To investigate the effect of polysaccharide sulfate 916 (PS916) on the production of nitric oxide (NO) in ECV304 cells induced by tumor necrosis factor-α (TNFα), interleukin-1β (IL-1β) and H2O2 in vitro. Methods Production of NO in ECV304 cells was measured by the Griess method and the proliferation of cells was tested by the MTT method. The activity of NO synthase was detected spectrophotometrically. Results Production of NO in ECV304 cells decreased after treatment with 40ng/ml IL-1β and 40ng/ml TNFα, but increased in the presence of H2O2 0.1mmol/L. PS916 significantly enhanced NO production in ECV304 cells in a dose-dependent manner in the TNFα and IL-1β treated groups and decreased it in the H2O2 treated group. Proliferation of ECV304 cells was inhibited by TNFα and H2O2 and no effect was found in the IL-1β treated group. PS916 increased the proliferation of cells treated with TNFα and H2O2 dose-dependently. In vitro, PS916 has no effect on the activity of NO synthase.Conclusion PS916 has a protective effect on ECV304 cells exposed to IL-1β, TNFα and H2O2.