摘要目的研究红毛五加多糖对人胃癌细胞SGC-7901的诱导凋亡作用.方法采用细胞增殖法来研究时间-效应和剂量-效应.采用超微形态结构,凝胶电泳技术和流式细胞术(FCM)对细胞凋亡进行分析.结果 1.0×10-4mol/L AGP显著抑制了胃癌细胞的增殖. 胃癌细胞在1.0×10-4 m ol/L AGPⅣ作用36 h后,癌细胞浓缩、深染,胞膜起泡,致密的染色质沿核膜下凝聚,有凋亡小体形成;细胞DNA电泳呈现梯状带型;细胞周期分析G1峰前出现明显的细胞凋亡峰,定量分析凋亡细胞在1.0×10-4 mol/L AGPⅥ作用72小时发生率为62.6%.细胞周期分析表明四种红毛五加多糖均使细胞周期停滞在G0/G1期.结论 AGP对胃癌细胞的增殖抑制作用具有剂量-效应和时间-效应关系.AGP可诱导胃癌细胞凋亡 .AGPⅡ可能是AGPⅣ的主要有效成份.

abstractsObjective To study the inhibitory effect of Acanthopanax giraldii Harms Var. Hispidus H oo polysaccharides (AGP) on SGC-7901 gastric cancer cells and its possible m echanism. Methods Cell doubling time analysis, colony forming assay and MTT assay were adopted to study the inhibitory effect and its characteristics. We also analyzed the amou nt of protein expressed by oncogenes, antioncogenes and cell factors using flow cytometric analysis.Results AGP inhibited the proliferation of SGC-7901 cells and cell colony forming abili ty. AGP did not inhibit the viability and function of lymphocytes of periphera l blood in healthy subjects and human embryonic tenocytes, except for the highes t dosage of AGP (P<0.05), which slightly inhibited the viability and functi on of the two types of normal cells. AGP inhibited the viability and function of SGC-7901 cells, except for the lowest dosages of AGPⅠ and AGPⅢ. There wa s a dose-effect relationship between the dosage of the AGP and SGC-7901 cells . The effect of the AGP at the molecular level was associated with the low prot ein expression of the c-myc and bcl-2 genes and the high protein expression of the p53, bax, fas and fas-L genes, as well as the cell factor TGFβ1. The i nhibitory effect of AGP was weaker than that of CDDP, but was stronger than that of Vitamine C. Conclusions Acanthopanax giraldii Harms Var. Hispidus Hoo polysaccharides selectively i nhibited the proliferation, the colony forming ability, and the viability and fu nction of human gastric cancer cells through the low protein expression of c-my c, bcl-2 and the high protein expression of p53, fas, fas-L and the cell facto r TGFβ1. The different inhibitory characteristics on the normal cells and c ancer cells are possibly caused by gene and the cell factor expressions.