摘要目的探讨产志贺样毒素大肠埃希菌(SLTEC)在小儿腹泻中的病原学地位. 方法根据肠出血性大肠埃希菌(EHEC)O157:H7 SLT1、SLT2、eaeA基因片段设计了3对引物,采用PCR法检测大肠埃希菌中SLT1、SLT2、eaeA毒力基因. 结果 29株标准致泻大肠埃希菌(EHEC、EPEC、ETEC)和10株其它肠道病原菌中,只有1株EHEC检出SLT1、SLT2、eaeA毒力基因,其它均为阴性.从1032例腹泻患儿粪便中分离的474株未定型大肠埃希菌中,检出SLT1 20株(4.2%), SLT2 7株(1.5%);74株产志贺样毒素侵袭性大肠埃希菌(ESIEC)中检出SLT1 15株(20.3%),SLT2 5株(6.8%).结论 SLTEC是太原地区引起小儿腹泻的一种重要的致泻病原菌.

abstractsObjective To evaluate the etiology of Shiga-like toxin-producing Escherichia coli (SLTEC) in children with diarrhea. Methods We designed and synthesized 3 pairs of primers located in the SLT1, SLT2, and eaeA genes of enterohaemorrhagic Escherichia coli (EHEC), while the virulent genes SLT1, SLT2, and eaeA from E.coli species were amplified by polymerase chain reaction (PCR). Results One strain of EHEC with SLT1, SLT2, and eaeA in 29 reference strains of diarrhea-causing E.coli (DCEC) and 10 strains of other enterobacteria detected by PCR had positive reactions, while all other DCEC and enterobacteria were negative. Of 474 strains of E. coli isolated from 1032 children with diarrhea and detected by PCR, 20 strains of SLT1 producing E. coli (4.2%) positive, and 7 strains of SLT2 producing E.coli (1.5%) positive; while of 74 strains of entero-SLTs-producing and invasive Escherichia coli (ESIEC), 15 strains of SLT1 (20.3%) and 5 strains of SLT2 (6.8%) were positive. Conclusion Shiga-like toxin E. coli has been identified as a major etiologic agent of children with diarrhea in Taiyuan, China.