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目的探讨内质网分子伴侣GRP94在核酸和蛋白水平的表达与人肺癌的关系.方法采用逆转录聚合酶链反应(RT-PCR)、免疫组化方法和/或Western 杂交,检测54例肺癌患者癌组织及癌旁正常肺组织GRP94在核酸和蛋白水平的表达.结果在肺癌组织及癌旁正常肺组织中均可检测到GRP94的表达,但肺癌组织GRP94在核酸和蛋白水平的表达均明显高于癌旁正常肺组织,肺癌组织GRP94 mRNA相对水平为3.48±2.06,GRP94蛋白水平免疫组化染色为++-+++,Western 杂交为256.7±80.6;而癌旁正常肺组织GRP94 mRNA相对水平为2.01±1.83,GRP94蛋白水平为+-++和108.1±42.3,二者存在显著差异(P<0.05).而且GRP94水平高低与肺癌分化程度及临床分期呈明显相关性,低分化组明显高于中-高分化组(P<0.05),临床Ⅲ期明显高于临床Ⅰ、Ⅱ期(P<0.05).但GRP94的表达与病理类型间未见明显相关性. 结论 GRP94的表达与肺癌的发生及发展密切相关.GRP94可能作为一种新的生物标记物以协助判断肺癌的分化程度和临床分期.

Objective To investigate the relationship between the expression of glucose regulated protein 94 (GRP94) at the level of mRNA and protein in vivo and in human lung cancer. Methods RT-PCR, immunohistochemistry and/or Western blot were used in 54 cases of lung cancer tissues and corresponding normal lung tissues.Results There was a significant overexpression of GRP94 mRNA and protein in lung cancer tissues as compared with lung normal tissues. In lung cancer tissue, the relative level of GRP94 mRNA as evaluated by RT-PCR was 3.48±2.06, the level of GRP94 protein as evaluated by immunohistochemistry was ++ to +++, and by Western blot was 256.7±80.6. In lung normal tissue, the relative level of GRP94 mRNA was 2.01±1.83, the level of GRP94 protein was + to ++ and 108.1±42.3. The differences in expression of GRP94 between the two tissues were significant (P<0.05). Furthermore, the over-expression of GRP94 in the lung cancer tissues was correlated to grade of differentiation and stage of tumors. There was stronger expression in poor-differentiated tumors than in mild-to-high differentiated tumors (P<0.05). There was also a stronger expression in stage Ⅲ than in stage Ⅰ and Ⅱ tumors (P<0.05). No statistically significant difference was found among various pathological types of tumors.Conclusion GRP94 was related with the occurrence, differentiation and progress of human lung cancer. Ascertaining the levels of GRP94 mRNA and protein may be valuable in evaluating the grade of differentiation and clinical stage of human lung cancer.