尿液中端粒酶活性在尿路上皮癌的诊断及监测复发中的意义
Telomerase activity in urine in diagnosis and recurrence surveillance of urothelial carcinoma
目的探讨尿液中端粒酶活性在尿路上皮癌的诊断和监测复发中的意义.方法采用PCR-ELISA法对54例尿路上皮癌尿液中端粒酶活性进行了检测,并观察了其中23例术后尿液中端粒酶活性的变化,以46例良性泌尿系统疾病患者作为对照组.结果尿路上皮癌病人尿液中端粒酶活性明显增高(P<0.001),肿瘤切除后恢复正常,在膀胱镜发现肿瘤复发前再次上升;随着肿瘤恶性程度的增加尿液中端粒酶活性逐渐升高;术前尿液中端粒酶活性与早期复发无关.结论尿液中端粒酶活性的检测在尿路上皮癌的诊断中有参考价值,与肿瘤恶性程度有关,敏感度高于膀胱镜检查,可能成为监测尿路上皮癌复发的重要辅助手段.
更多Objective To investigate the significance of telomerase activity in urine in the diagnosis and recurrence surveillance of urothelial carcinoma. Methods Telomerase activity in urine of 54 cases of urothelial carcinoma (urothelial carcinoma group) was estimated by polymerase chain reaction-enzyme-linked immunosorbent assay, and monitored continuously in 23 cases after tumor removal. 46 patients with benign urological diseases were also included as the control group. Results The telomerase activity in urine of patients with urothelial carcinoma increased significantly as compared with the control group(P<0.001), decreased into the normal range after tumor removal, and rose again upon intravesical tumor recurrence before the screening of recurrent tumors by cystoscopy. The higher the grade of tumor, the higher the telomerase activity; no correlation could be found between the preoperative level of telomerase activity in urine and recurrence. Conclusions The examination of telomerase activity in urine is helpful in the diagnosis of urothelial carcinoma and may be related to the differentiation degree of tumors. Its sensitivity is higher than that of cystoscopic examination and may become an important ancillary method in the screening of urothelial carcinoma recurrence.
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