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Endothelial cell chimerism by fluorescence in situ hybridization in gender mismatched renal allograft biopsies

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Background The blood vessels of a transplanted organ are the interface between donor and recipient. The endothelium in the blood vessels is thought to be the major target for graft rejection. Endothelial cells of a transplanted organ can be of recipient origin after transplantation. In this study, we tested whether endothelial chimerism correlated with the graft rejection and cold ischemia.Methods We studied the biopsy samples from 34 renal transplants of female recipients who received the kidney from a male donor for the presence of endothelial cells of recipient origin. We examined the tissue sections of renal biopsy samples by fluorescence in situ hybridization (FISH) for the presence of endothelial cells containing two X chromosomes using a biotinylated Y chromosome probe and digoxigenin labelled X chromosome probe, and then analyzed the relationship between the endothelial cell chimerism and the rejection and cold ischemia.Results Endothelial chimerism was common and irrespective of rejections (P>0.05). The cold ischemic time of chimerism group was longer than no chimerism group ((14.83±4.03) hours vs (11.27±3.87) hours, P<0.05).Conclusions There is no correlation between the percentage of recipient endothelial cells in vascular endothelial cells and the type of graft rejection. The endothelium damaged by ischemic injury might be repaired by the endothelial cells from the recipient.

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作者单位: Transplantation Centre of PLA, Second Affiliated Hospital of PLA General Hospital, Beijing 100091, China [1] Department of Urology, Institute of Urology, Peking University,Beijing 100034, China [2] Department of Pathology, Peking Union Medical College Hospital,Peking Union Medical College & Chinese Academy of Medical Sciences, Beijing 100730, China [3] Department of Pathology, Peking University Health Science Center,Beijing 100083, China [4]
期刊: 《中华医学杂志(英文版)》2007年120卷10期 859-862页 SCIMEDLINEISTICCSCDBP
分类号: R6
栏目名称: ORIGINAL ARTICLES
发布时间: 2008-03-03
基金项目:
the grant from the Capital Medical Progressing and Research Funds
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