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Overexpression of cyclin L2 induces apoptosis and cell-cycle arrest in human lung cancer cells

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Background Uncontrolled cell division is one of the hallmarks of tumor growth. Researches have been focused on numerous molecules involved in this process. Cyclins are critical regulatory proteins of cell cycle progression and/or transcription. The present study aimed to investigate the anti-proliferative effect of cyclin L2, and to define its growth regulatory mechanisms using human lung adenocarcinoma cell line A549.Methods Human cyclin L2 was transfected into human lung adenocarcinoma cells (A549 cell), and was expressed in a mammalian expression vector pcDNA3.1. The effects and mechanisms of the cyclin L2 in cell growth, cell cycle analysis and apoptosis were studied by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), flow cytometry or Western blot, respectively.Results Overexpression of cyclin L2 inhibited the growth of A549 cells. Cell cycle analysis in cells transfected with pCCNL2 revealed an increment in proportion in G0/G1 phase ((68.07 ± 4.2)%) in contrast to (60.39 ± 2.82)% of the cells transfected with mock vector. Apoptosis occurred in (7.25 ± 0.98)% cells transfected with pCCNL2, as compared with (1.25 ± 0.21)% of the mock vector control group. Cyclin L2-induced-G0/G1 arrest and apoptosis involved upregulation of caspase-3 and downregulation of Bcl-2 and survivin.Conclusion The results indicate that overexpression of cyclin L2 protein may promote efficient growth inhibition of human lung adenocarcinoma cells by inducing G0/G1 cell cycle arrest and apoptosis.

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作者单位: Department of Gastrointestinal Oncology, Tianjin Medical University Cancer Institute and Hospital, Tianjin 300060, China [1] Nanjing Medical University, Nanjing 210029, China [2] Institute of Immunology, Second Military Medical University,Shanghai 200433, China [3]
期刊: 《中华医学杂志(英文版)》2007年120卷10期 905-909页 SCIMEDLINEISTICCSCDBP
分类号: R73
栏目名称: ORIGINAL ARTICLES
发布时间: 2008-03-03
基金项目:
国家自然科学基金 Educational Foundation of Tianjin
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