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An evaluation of the mechanism of ABCA7 on cellular lipid release in ABCA7-HEC293 cell

摘要:

Background ABCA7 is a member of the ABCA subfamily that shows a high degree of homology to ABCA1 and,like ABCA1,mediates cellular cholesterol and phospholipid release by apolipoproteins when transfected in vitro.However,expression of ABCA7 has been shown to be downregulated by increased cellular cholesterol while ABCA1 was upregulated.Methods The underlying mechanism for this effect was examined in ABCA1 or ABCA7-transfected HEC293.Lipid content in the medium and cells was determined by enzymatic assays.Gene expression was quantitated by real time PCR,and protein content was determined by Western blotting.Results While ABCA7 mRNA was decreased by 25-hydroxycholesterol treatment,ABCA1 was apparently increased.Treatment with the synthetic LXR agonist T0901317 (T09) upregulated ABCA1 expression and apoAI-mediated cellular lipid release in ABCA1-transfected HEC293 cells,but ABCA7 expression and cellular lipid release in ABCA7-transfected HEC293 cells showed no obvious changes.Conclusion The ABCA7 gene is regulated by sterol in a direction opposite to that of ABCA1.

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作者单位: Department of Molecular Orthopaedics, Beijing Institute of Traumatology and Orthopaedics, Beijing 100035, China ;Beijing Institute of Heart Lung and Blood Vessel Diseases, Beijing 100035, China [1] Department of Molecular Orthopaedics, Beijing Institute of Traumatology and Orthopaedics, Beijing 100035, China [2]
期刊: 《中华医学杂志(英文版)》2013年126卷2期 306-310页 SCIMEDLINEISTICCSCDBP
栏目名称: ORIGINAL ARTICLES
DOI: 10.3760/cma.j.issn.0366-6999.20123502
发布时间: 2013-04-19
基金项目:
a grant from the National Natural Science Foundation of China
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