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Systematic analysis of protein expression in Candida albicans exposed to farnesol

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Backsround:The phenotypic switching of Candida spp.plays an important role in the development of vulvovaginal candidiasis (WC).Farnesol,as a quorum-sensing molecule in Candida albicans,has the ability to prevent yeast-to-hyphal conversion in vitro.However,the mechanism underlying this ability is unclear.This study aimed to investigate changes in protein levels to better understand how farnesol impacts processes contributing to WC.Methods:The isobaric tag for relative and absolute quantitation technique was used to detect protein expression in C.albicans strain SC5314 (ATCC MYA-2876) with or without farnesol exposure.Proteins with a threshold fold change greater than 1.5 were screened and considered differentially expressed proteins.All the altered proteins were analyzed using Gene Ontology annotation,Kyoto Encyclopedia of Genes and Genomes (KEGG) annotation,and metabolic pathway annotation.Results:Between the farnesol-exposed group and the farnesol-unexposd group,we detected 297 altered proteins among all 2047 tested proteins based on a threshold fold change of more than 1.5 (P < 0.05).Eighty-seven of the 297 altered proteins exhibited metabolic enzyme activity and participated in 85 metabolic pathways according to KEGG pathway analysis.Most of these metabolic pathways were associated with central carbon metabolism processes.In the sterol synthesis pathway,which involves the synthesis of farnesol,ERG25 (methylsterol monooxygenase) and ERG4 (delta 24(24(1))-sterol reductase) were both downregulated in the farnesol-exposed group.All six altered proteases associated with the oxidative phosphorylation process were downregulated in the farnesol-exposed group relative to the farnesol-unexposed group.Conclusions:The mechanisms underlying farnesol-induced phenotype switching involves the adjustment of metabolic activities and epigenetic modification.Exogenous farnesol had an evident,but non-deterministic effect on the synthesis of ergosterol.The potential drug activity of farnesol warrants further investigation.

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作者单位: Department of Obstetrics and Gynecology, Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing 100026, China [1]
期刊: 《中华医学杂志(英文版)》2019年132卷19期 2348-2353页 SCIMEDLINEISTICCSCDBP
栏目名称: Original Articles
DOI: 10.1097/CM9.0000000000000420
发布时间: 2019-11-19
基金项目:
a grant from the National Natural Science Foundation of China
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