摘要目的 应用声脉冲辐射力成像(ARFI)技术观察家兔肌肉损伤后不同观察时间点肌肉组织修复的动态变化情况,并探讨其临床应用价值.方法 根据不同观察时间,将16只健康家兔随机分为4组,即损伤前和损伤后1d、7d、14d.用自制的重力锤建立兔肌肉损伤模型,应用ARFI观察腓肠肌声触诊组织定量(VTQ)测值的变化.实验结束后将兔处死,取肌肉组织行病理检查.结果 ARFI示肌肉损伤后各组VTQ值明显高于正常肌肉组织,差异均具有统计学意义(P＜0.01),损伤后1d、7d、14 d组间差异均具有统计学意义(P＜0.01).病理检查结果示损伤前肌纤维连续,未见明显肿胀、出血；损伤后1d,肌纤维断裂、明显肿胀、出血、充血；损伤后7d,肌细胞大面积坏死,大量的钙盐沉积；损伤后14d,大量的纤维细胞增生,钙盐沉积明显减少.超声弹性成像与病理结果相一致.结论 ARFI可直观、无创地评价肌肉损伤后不同时间点肌肉组织修复变化,为肌肉损伤后诊断和治疗提供客观依据.

abstractsObjective To observe the dynamic change of muscle tissue restoration after damaging at different observation time through acoustic radiation force impulse(ARFI).Methods According to different observation time,16 healthy rabbits were randomly divided into four groups,including before injury,1 day after injury,7 day after injury,14 day after injury.A homemade gravity hammer was used to establish damage model of rabbit gastrocnemius,then applied ARFI to observe changes of virtual touch tissue quantification (VTQ) of gastrocnemius.All the rabbits were executed after measurement,then the pathological changes of muscle tissue were observed under microscope.Results The VTQ of damaged muscle group were significantly higher than that of nomal muscle group( P ＜0.01),and VTQ of 1 day,7day,14 day group after injuried had significant difference between two groups (P ＜ 0.01).Through pathological examination,normal musule fibers were continual and there were not swelling and bleeding.One days after injury,muscle fibers were fractured,swollen,bleeding and congestive.7 days after injury,large areas of muscle cells were necrotic,and amounts of calcium salt depositsed.14 days after injury,lots of fiber cells proliferated,and the deposits of calcium obviously reduced.The results of ultrasound elastography were consistent with these of pathological.Conclusions ARFI can direct,noninvasively evaluate the changes of muscle tissue restoration after different time of injury,and provide objective basis for diagnosis and treatment.