摘要目的 观察低浓度一氧化碳(CO)吸入对脂多糖(LPS)诱导大鼠多器官(肺、肠)损伤的影响.方法 72只雄性SD大鼠随机均分为对照(A)、单纯CO吸入(B)、LPS注入(c)和LPS注入+c0吸入(D)4组,静脉注入5 mg/kg体质量LPS或等容量生理盐水,1h后,A、C组吸入室内空气,B、D组吸入2.5 X 10-4体积分数的CO.观察1、3、6 h后批次放血处死,取小肠和肺,酶联免疫吸附法测定肺肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL*6)、白细胞介素-10(IL-10)、肠血小板活化因子(PAF)、细胞间黏附分子-l(ICAM-1)含量,化学比色法测定肺、肠组织丙二醛(MDA)含量、髓过氧化物酶(MPO)及超氧化物歧化酶(SOD)活性,流式细胞仪检测细胞凋亡率;半定量逆转录聚合酶联反应检测血红素加氧酶-1(HO-1)mRNA,光镜下观察组织形态学变化.结果 TNF-α、IL-6、PAF、ICAM-1、MDA、MPO及细胞凋亡率的组间相应时间点比较,C组高于A、B组(P值均<0.05),D组低于C组但高于A、B组(P值均<0.05);IL-10和SOD组间相应时间点比较,C组低于A、B组(P值均<0.05),D组高于C组但低于A、B组(P值均<0.05);C组肺、肠损伤严重,D组损伤轻于C组但重于A、B组.A组与B组间及组内各时间点比较,上述指标差异均无统计学意义(P值均>0.05).结论 低浓度CO吸入通过调控氧化/抗氧化反应、促炎/抗炎反应,可抑制细胞凋亡,减轻脂多糖诱导的多器官损伤.

abstractsObjective To investigate effects of low concentration carbon monoxide(CO) inhalation onrat with multiple organ(lung and intestine) injury induced by lipopolysaccharide(LPS).Methods Seventy-two male Sprague-Dawley rats were randomly divided into 4 groups:control(A),CO inhalation alone(B),LPS injection(C) and LPS injection+CO inhalation(D)groups.After 1h of LPS or an equal volume ofnormal saline administration by tail vein.the rats in C group exposed to room air after an intravenous injection of 5 mg/kg body weight LPS.the rats in B group were exposed to 2.5×10-4 CO simply,the rats in D group were intravenously injected 5 mg/kg body weight LPS and exposed to 2.5×10-4 CO continuously,and the rats in A grouP were also exposed to room air.All rats were sacrificed by exsanguination at 1,3 and 6 hours after observation.Lung and intestine tissues were homogenized for determination the levels of lung tumor necrosis factor-α(TNF-α),interlukin-6(IL-6),interlukin-10(IL-10),intestine platelet activator(PAF),intercellular adhesion molecule-1(ICAM-1)and IL-10 with enzyme-lined immunosorbent assay,the maleic dialdehyde (MDA)content.myeloperoxidase(MPO) and superoxide dismutase (SOD) activity with chemical colorimetric method.the extent of cell apoptosis with flow cytometery,the expression level of the heme oxygenase-1(HO-1) gene with semi-quantitative reverse transcription-polymerase chain reaction and the histology with light microscopy.Results Compared with both A and B groups at the same time point,the TNF-α,IL-6,PAF, ICAM-1,MDA,MPO and cell apoptosis rate of C group were increased(all P＜0.05),while IL-10 and SOD decreased(all P＜0.05),lung and intestine damage was remarkable.Moreover,with prolonging of time,the cell apoptosis rate in C group increased.As compared with C group at the same time point,the TNF-α,IL-6, PAF,ICAM-1,MDA,MPO and cell apoptosis rate of D group was decreased(all P＜0.05),while IL-10 and SOD increased(all P＜0.05),organ damage was obviously ameliorated.There were no statistical differences between A and B groups and between the different time point of same one group.Conclusions Low concentration CO inhalation could protect rat lung and intestine from LPS-induced injury via suppressing cell apoptosis,regulating the disorder of oxidant-antioxidant and imflammation-antiinflammation.