摘要目的 探讨早期高压氧、低气压干预对低气压环境大鼠降钙素基因相天蛋白(calcitonin gene related protein,CGRP)和内皮素(endothelin,ET)的影响,对比研究两种方法的作用.方法 62只Wistar大鼠随机分成6组:(1)对照组,(2)海拔3800 m(2 h)组,(3)海拔3800 m(3 d)组,(4)海拔3800 m(7 d)组,(5)高压氧f预组,(6)低气压环境干预组.首先把(2)、(3)、(4)实验组大鼠置于低压氧舱中,匀速减压使舱内压力南海平面0.1 MPa降至0.062 MPa,相当于海拔3800 m的高原低气压环境,在2 h、3 d、7 d时间点断头取血备检;对(5)组大鼠先期给予高压氧处理(0.2 MPa,1次/d,吸氧60mjn,连续5 d),第6天,再置于低压氧舱中匀速减压至0.062 MPa,2 h后断头取血备检;对(6)组大鼠先期给予低气压缺氧处理(0.062 MPa,1次/d,2 h/次,连续5 d),第6天,再置于低压氧舱中匀速减压至0.062 MPa,2 h后断头取血备检;将上述不同处理方法所得血样离心取上清,放免法测定CCRP、ET浓度.结果 与对照组比较,CGRP浓度随着大鼠在低气压环境中的时间延长有逐渐减少趋势,但差异无统计学意义,高压氧预处理对CGRP无影响,低气压缺氧预处理虽然使CGRP浓度下降趋势更明显,但差异无统计学意义.ET浓度随着时间的延长有逐渐增加趋势,但也无统计学意义.高压氧[(305.26±39.66)μg/L]、低气压缺氧预处理[(309.18±25.07)μg/L]均使ET浓度明显升高,差异有统计学意义(P<0.05).结论 高压氧、低气压缺氧早期干预主要影响大鼠ET的变化,高压氧预处理可能具有增强机体高原适应能力的作用.

abstractsObjective To investigate effects of early hyperbaric oxygen and hypobaric intervention on the levels of calcitonin gene related protein (CGRP) and endothelin ( ET) in rats in hypobaric environment, and to compare the effects of two methods. Methods The experimental Wistar rats were randomly divided into six groups: (1) Control group, (2) 3800 m(2 h) group, (3) 3800 m (3 d) group, (4) 3800 m (7 d) group, (5) Hyperbaric oxygen intervention group, (6) Hypobaric environment intervention group. Firstly, the rats in the second, third and fourth groups were placed in a hypobaric oxygen chamber, in which the pressure was decreased at equal speed from 0. 1 MPa to 0. 062 MPa, which corresponded the pressure at the 3800 m altitude. After two hours and the 3rd, 7th days, the rats were decapitated to obtain blood for later examination. Secondly, the rats in the fifth group received hyperbaric oxygen treatment (0. 2 MPa, once per day, oxygen inhalation for 60 minutes, successively 5 days). On the sixth day, the rats were placed in a hypobaric oxygen chamber, in which the pressure was decreased at equal speed to 0. 062 MPa. After two hours, the rats were decapitated to obtain blood for later examination. Thirdly, the rats in the sixth group received hypobaric hypoxia treatment (0.062 MPa, once per day, 2 h once, for successively 5 days). On the sixth day, the rats were placed in a hypobarie oxygen chamber, in which the pressure was decreased from equal speed to 0. 062 MPa. After two hours, the rats were decapitated to obtain blood for later examination. The blood samples obtained from above different treatment methods were centrifuged to obtain the supernatant, and the levels of CGRP and ET were measured by radioimmunology method. Results Compared with control group, CGRP level decreased in tendency with time extension, but there was no significant difference between of them. The hyperbaric oxygen treatment had no effect on CGRP level, but hypobaric hypoxia pretreatment can decrease CGRP level more obviously, but there was no significant difference between of them either. ET level increased in tendency with time extension, but there was no significant difference between of them. However, EF level significantly increased (P<0.05) after hyperbaric oxygen[ (305. 26 ±39. 66) μg/L] and hypobaric hypoxia pretreatment [ (309. 18 ± 25. 07) μg/L]. Conclusions Early intervention of hyperbaric oxygen and hypobarie hypoxia mainly influences ET level in rats, and hyperbaric oxygen pretreatment might enhance body adaptive capacity for plateau.