摘要目的 通过同一实验条件下的重复实验,比较目前常用的大鼠运动病3种判断指标的准确性和可重复性.方法 用Crampton装置给予雄性SD大鼠加速度刺激,每天1 h,连续3 d,观察空白对照组、东莨菪碱+加速度刺激组和生理盐水+加速度刺激组大鼠高岭土摄入量、晕反应指数及自发活动值的变化.结果 3次实验中,大鼠接受加速度刺激后,与对照组相比,高岭土摄人量的变异较大;晕反应指数均显著升高,给予东莨菪碱可以减小升高幅度;大鼠自发活动差异均无统计学意义(P>0.05).结论 在本实验条件下,晕反应指数作为大鼠运动病判断指标具有较好的准确性和可重复性.

abstractsObjective To compare the accuracy and reproducibility of 3 commonly used indices of rat motion sickness through repeated experiments under the same conditions. Methods Male SD rats were subjected to acceleration 1 hour a day for successive 3 days by the Crampton device. Then, observations were made on changes in kaolin consumption, motion sickness (MS) index and spontaneous activities of the control group, the stimulation group with saline, and the stimulation group with scopolamine. Results Experiments showed that the stimulation groups varied greatly in kaolin consumption, MS index increased significantly, when compared with the control group. It was noted that MS index could be attenuated by seopelamine. However, no statistical significance could be seen in spontaneous activity of rats. Conclusions Results showed that MS as an index for its assessment had better accuracy and reproducibility under the same experimental conditions.