摘要目的 研究高压氧(hyperbaric oxygen,HBO)对维生素C药代动力学的影响.方法 采用数字表法将240只小鼠随机分为2组,每组120只,2组均按3g/kg剂量单次灌服维生素C注射液.HBO组灌服后立即进入高压氧舱,稳压(0.10 MPa)吸纯氧20 min,升压、减压各5 min;对照组仅单次灌服维生素C注射液.2组在给药前及给药后不同时间点各取10只小鼠,摘眼珠取血,采用高效液相色谱法测定血浆中维生素C的浓度,计算药代动力学参数.结果 HBO组赤池信息量准则(AIC)、血浆半衰期(t1/2)、达峰时间(tpeak)、峰浓度(cmax)、平均血药浓度-时间曲线下面积(AUC)分别为-26.4、5.4h、0.8h、17.9 mg/L、126.2(mg· h)/L;对照组AIC、t1/2、tpeak、cmax、AUC分别为-3.8、11.6h、1.1h、11.2mg/L、155.5 (mg·h)/L.结论 HBO可以明显加快小鼠体内维生素C的吸收,对药代动力学参数有明显的影响.

abstractsObjective To investigate the effects of hyperbaric oxygen (HBO) on the pharmacokinetics of vitamin C.Methods Two hundred and forty mice were randomly divided into 2 groups,the HBO group and the control group,each consisting of 120 animals.The 2 groups of animals received gavage of vitamin C with a single-dose of 3g/kg.The animals in the HBO group were immediately put into the hyperbaric oxygen chamber and maintained at a pressure of 0.1 MPa for a duration of 20 min.The time used for compression and decompression was all 5 min.The animals in the control group were just given a single gavage of vitamin C.Ten animals from each group were chosen at different time points both before and after drug administration,for collection of blood samples from the eyeballs.Concentrations of vitamin C in serum were monitored with high performance liquid chromatography,and pharmacokinetic indices were calculated accordingly.Results The main pharmacokinetic parameters of the HBO group were as follows:AIC ( - 26.40 ),t1/2 ( 5.4 h),Tpeak (0.8 h),Cmas ( 17.9 mg/L),AUC[126.2( mg · h)/L],while the corresponding results of the control group were AIC ( - 3.8 ),t1/2 ( 11.6h),Tpeak ( 1.1 h),Cmax ( 11.2 mg/L),AUC [155.5 ( mg · h)/L ].Conclusions HBO could obviously accelerate the absorption of vitamin C and seemed to have significant effect on pharmacokinetic parameters.