摘要目的 构建高效降解海洋柴油污染物的优势菌群,并对该菌群的降解性能进行研究,为海洋柴油污染物的生物修复奠定基础.方法 从柴油污染海水中富集、筛选得到4株柴油高效降解菌株,分别命名为S1、A1、A2、A3,以此为基础进行组合优化试验,对其降解特性进行研究.利用紫外分光光度法测定降解率,GC - MS方法分析菌群对柴油组分的降解.结果 各种菌株组合中,以S1+ A1+A2+A3组合为最优菌群,菌株接种比例为1∶1∶2∶1时降解效率最高,该菌群在第7天时对柴油的降解率达到71.2％,并能降解柴油中所含的碳链长度在C11～C19的所有烷烃组分.结论 构建的优势菌群对柴油具有较强的降解作用,底物范围广,有望应用于海洋柴油污染的生物修复.

abstractsObjective To construct a superior marine diesel-degrading bacterial consortium,to study its degradation features,and ultimately to provide an evidence for the bioremediation of marine diesel pollution.Methods Four bacterial strains named S1,A1,A2,A3 were isolated from the offshore diesel polluted seawater,and then,combination and optimization tests were made.Studies were also made on the degradation features of the bacterial consortium.Rate of degradation was measured with ultraviolet spectrometer.Diesel degrading capacity of the bacterial consortium was analyzed with GC-MS.Results Of the four optimized strains in the bacterial consortium,the combination of S1 + A1 + A2 + A3 was the most superior,and the rate of degradation was the highest,when the proportion of the inoculated strains was 1 ∶ 1 ∶ 2∶ 1.The degradation rate was 71.2％ on the 7th day.The bacterial consortium was capable of degrading all the alkanes in the diesel from C11-C19.Conclusions The constructed superior bacterial consortium seemed to have high diesel-degrading capacity and a wide range of substrates.It was expected that it could be applied to bioremediation of marine pollution.