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目的 探讨高压氧(hyperbaric oxygen,HBO)联合褪黑素对胃腺癌SGC7901细胞分化的影响,并探讨可能的分子机制.方法 将处于对数生长期的胃腺癌SGC7901细胞分为对照组、单独褪黑素处理组和高压氧联合褪黑素组.采用流式细胞术检测各组分化细胞比例,Western blot检测各组分化相关endocan蛋白的表达情况.结果 褪黑素处理胃腺癌SGC7901细胞,使处于细胞间期的细胞比例增加[单独褪黑素处理组(74.35±0.88)％,对照组(73.12±1.01)％],并使endocan的表达上调单独[褪黑素处理组(2.14 ±0.12),对照组(1.99±0.04)],但差异均无统计学意义(P＞0.05),而同时给予HBO处理时,细胞分裂间期细胞的比例[HBO联合褪黑素组(76.86％±1.13％)]和endocan的表达[HBO联合褪黑素组(2.57 ±0.03)]都进一步增加,与对照组和褪黑素单独处理组比较差异均有统计学意义(P＜0.05).结论 HBO处理明显促进褪黑素对胃癌细胞SGC7901的诱导分化作用,其机制可能与HBO促进褪黑素上调endocan表达有关.

Objective To investigate the effects of hyperbaric oxygen (HBO) on the melatonin enhancement of the differentiation of gastric cancer cells SGC7901 and also to explore the possible mechanism involved.Methods The SGC7901 cells at logarithmic differentiation stage were divided into the control group,the melatonin treatment group and the HBO coupled with melatonin group.The rate of cell differentiation was detected by flow cytemctry,and endocan expression was measured by using Western blot assay.Results SGC7901 cells treated with melatonin could enhance the rate of the cells at differential stage (the rate of the melatonin treatment group was 74.35％ ±0.88％,while that of the control group was 73.12％ ± 1.01％).Melatonin treatment could also up-regulate the expression level of endocan cells (the expression level of the melatonin treatment group was 2.14 ± 0.12,while that of the control group was 1.99 ± 0.04).However,when treated with HBO + melatonin,both the rate of the cells at the differential stage (the rate of the cells of the HBO coupled with melatonin group was 76.86％ ± 1.13％) and the expression of endocan cells (the rate of endocan cells of the HBO coupled with melatonin group was 2.57 ± 0.03) were further enhanced,and statistical significance could be noted,when compared with those of the control group (P ＜ 0.05).Conclusions HBO treatment could obviously enhance the effect of melatonin on the differentiation of gastric cancer cells SGC7901,the mechanism of which might be associated with the up-regulation of endocan cells by melatonin.