摘要目的 探讨高压氧(hyperbaric oxygen,HBO)调控PI3K/AKT通路对大鼠脊髓损伤(spinal cord injury,SCI)后炎症反应的作用及其机制.方法 将30只大鼠按照随机数字表法分为HBO组、HBO联合PI3K组及LY294002(PI3K抑制剂)组,每组10只;另取40只按照SCI时间分为4组,每组10只,分别为损伤后1、5、10 d组及未损伤组(对照组).采用蛋白免疫印迹(Western Blotting)法和酶链免疫实验(ELISA)法检测SCI后1、5、10 d及HBO治疗后磷脂酰肌醇-3激酶(phosphoinositide 3-kinase,PI3K)、蛋白激酶B(protein kinase B,AKT)、p-PI3K、p-AKT、干扰素-γ(IFN-γ)、白细胞介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)的表达水平;采用ELISA法分别检测HBO组、HBO联合PI3K组、LY294002组及损伤,d组IFN-γ、IL-6、TNF-α的表达水平.结果 HBO治疗可显著提高大鼠SCI后PI3K、AKT、p-PI3K、p-AKT的表达水平,降低IFN-γ、IL-6、TNF-α的表达水平(均P＜0.05);此外,HBO组、LY294002组IFN-γ、IL-6、TNF-α的表达下降(P＜0.05);但过表达PI3K后,这种作用显著抑制(P＜0.05).结论 HBO通过调控PI3K/AKT通路显著抑制大鼠SCI后的炎症反应,表明HBO在SCI的临床治疗中发挥重要作用,尤其对于SCI后的炎症反应.

abstractsObjective To explore the effects of hyperbaric oxygen (HBO) on inflammatory response through PI3K/AKT pathway after spinal cord injury (SCI) in rats and investigate the possible mechanism involved.Methods Thirty rats were divided into the HBO group,the HBO combined with PI3K group and the LY294002 group,each consisting of 10 animals.In accordance with the time of spinal cord injury,another 40 rats were divided into 4 groups,i.e.the injury on day 1 group (or the day 1 group),the injury on day 5 group (or the day 5 group),the day 10 group and the control group (the non-injury group),each consisting of 10 animals.One day,5 and 10 days after spinal cord injury,as well as after HBO therapy,the expression levels of phosphoinositide 3-kinase (PI3K),protein kinase B (AKT),p-PI3K,p-AKT and IFN-γ,IL-6 and TNF-α were respectively detected by using Western Blotting and ELISA.The expression levels of IFN-γ,IL-6 and TNF-α in the HBO group,the HBO combined with PI3K group,the LY294002 group and the day one group were detected by using ELISA.Results HBO therapy could significantly increase the expression levels of PI3K,AKT,p-PI3K,p-AKT following spinal cord injury,and decrease the expression levels of IFN-γ,IL-6 and TNF-α(P ＜ 0.05).The expression levels of IFN-γ,IL-6 and TNF-α were decreased both in the HBO group and the LY294002 group,however,their expression levels were significantly depressed following over expression of PI3K (P ＜ 0.05).Conclusions HBO could significantly inhibit inflammation response after spinal cord injury in rats through the regulation of the PI3K/AKT pathways,indicating that HBO could play an important role in clinical treatment of spinal cord injury,especially in the case of inflammatory response after spinal cord injury.