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目的 检测运动病不同敏感性大鼠内耳加压素(arginine vasopressin,AVP)V2受体(V2 receptor,V2R)和水通道蛋白2(aquaporin 2,AQP2)在旋转刺激后表达的变化,探讨内耳AVP-V2R-AQP2信号通路与运动病的相关性.方法 采用大鼠运动病模型,根据旋转刺激诱发的条件性味觉厌恶程度和异食高岭土行为判断其运动病敏感性.采用Real-time PCR、Western-blot技术检测V2R mRNA与蛋白、AQP2 mRNA与蛋白在不同敏感性大鼠内耳表达水平及旋转刺激后的变化.结果 基础状态下,敏感组AQP2表达量高于不敏感组大鼠,而敏感组V2R表达量低于不敏感组大鼠;旋转刺激后,V2R表达量显著减少,而AQP2表达量显著增加;尾静脉注射V2R拮抗剂OPC-31260可以明显抑制旋转刺激诱发运动病,且AQP2表达量显著减少.结论 内耳AVP-V2R-AQP2信号通路的活动可能参与运动病的发生,使用V2受体拮抗剂抑制这一通路的活动可抗运动病.

Objective To observe changes in the expressions of vasopressin receptor 2 (V2R) and aquaporin 2 (AQP2) induced by rotary stimulation in the inner ear of rats with different susceptibilities to motion sickness, and to investigate involvement of the inner ear AVP -V2R-AQP2 signaling pathway in motion sickness.Methods The study was made by using the rat motion sickness model .The degree of conditioned taste aversion (CTA) and pica (kaolin intake) served as indices of susceptibility to motion sickness in rats . The levels of V2R and AQP2 in the inner ears and their changes in the expression after rotary stimulation were detected with Real-time PCR and Western-blot.Results At the basic state, the expression of AQP2 in the susceptible group was higher than that in the insusceptible group , however, the expression of V2R in the susceptible group was lower than that in the insusceptible group .Following rotary stimulation, the expression of AQP2 was increased significantly while the expression of V 2R was decreased instead.Injection of V2R antagonist OPC 31260 significantly inhibited motion sickness induced by rotary stimulation , and the amount of AQP2 expression was decreased considerably .Conclusions Research results revealed that the inner ear AVP -V2R-AQP2 signaling pathway might be involved in the development of motion sickness and the inhibition of the signaling pathway by V2R antagonist could reduce susceptibility of motion sickness .