摘要目的 探讨高压氧(HBO)处理对胰腺炎大鼠体内氧化应激及炎性因子水平的影响.方法 将80只SD成年雄性大鼠按照数字表法随机分成4组:对照组、假手术组、模型组和HBO组,每组20只.对模型组和HBO组大鼠进行胰腺炎造模;假手术组大鼠仅进行麻醉、开腹并且翻动肠管操作,随后进行腹腔缝合;对照组给予常规饲养.HBO处理后对各组大鼠胰腺进行病理评分,检测各组大鼠血清内细胞炎性因子IL-6、1L-10、IL-1β、TGF-α以及氧化应激因子MPO、MDA、ROS的表达水平.结果 模型组大鼠的病理评分(6.58±1.51)显著高于对照组(0.45±0.05)以及假手术组(0.46±0.04)(均P＜0.01).HBO组胰腺组织肿大以及炎性细胞的浸润,与模型组相比,其腹水较少,且出血点少,胰腺纹路也较模型组更清晰,其病理评分明显低于模型组(P＜0.01).与对照组和假手术组比较,模型组中IL-6、IL-1β、TNF-α、IL-10的表达水平显著升高,差异有统计学意义(P＜0.01);而HBO组中IL-6、IL-1β、TNF-α、IL-10较模型组的表达水平显著下调,差异有统计学意义(P＜0.05).与对照组和假手术组比较,模型组中MDA、MPO、ROS表达水平显著升高,差异有统计学意义(P＜0.01);而HBO组中MDA、MPO、ROS较模型组的表达水平显著下调,差异有统计学意义(P＜0.05).结论 HBO处理可显著降低胰腺炎大鼠体内的炎性反应以及氧化应激水平,减少大鼠胰腺腺泡的坏死和出血,从而有效改善大鼠胰腺炎症状.

abstractsObjective To investigate the effects of hyperbaric oxygen (HBO) on inflammatory factors and oxidative stress levels in the rats with pancreatitis.Methods Eighty SD adult male rats were randomly divided into 4 groups:the control group,the sham surgery group,the model group and the HBO oxygen group,with 20 rats in each group.Pancreatitis model was developed in the rats of the model and HBO groups.The rats in the sham surgery group were just anesthetized and abdomen was opened,and then the opened abdomen was sutured,while the animals in the control group were fed as routine.Following HBO treatment,pancreatic pathological scores of each group were evaluated,and the expression levels of inflammatory factors of IL-6,IL-10,IL-1β and TGF-α as well as oxidative stress factors of MPO,MDA,ROS in serum were detected.Results The pathological scores of the model group were significantly higher than those of the control group and the sham surgery group(6.58 ± 1.51 vs.0.45 ± 0.05,0.46 ± 0.04) (all P ＜ 0.01).Swelling of the pancreatic tissue and infiltration of inflammatory cells could clearly been seen in the rats of the HBO group.As compared with the model group,ascetic fluid level was less,with less hemorrhagic spots and clear pancreatic marbling.Furthermore,pathological scores were significantly higher than those of the model group (P ＜ 0.01).As compared with the control group and the sham surgery group,the expression levels of IL-6,IL-1β,TNF-α and IL-10 in the model group considerably elevated,and stastical significance could be seen when comparisons were made between them (P ＜ 0.01),while the expression levels of IL-6,IL-1β,TNF-α and IL-10 in the HBO group were noticeably up-regulated in the model group,also with stastical significance(P ＜ 0.01).As compared with the control group and the sham surgery group,the expression levels of MDA,MPO and ROS in the model group were obviously up-regulated,also with stastical significance (P ＜ 0.01),while the expression levels of MDA,MPO and ROS in the HBO group were significantly down-regulated as compared with the model group,also with statistical significance (P ＜ 0.05).Conclusion HBO treatment could significantly reduce the inflammatory reaction and oxidative stress levels in the rats with pancreatitis,and reduce necrosis and hemorrhage of pancreatic acinus,thus effectively improving pancreatic symptoms of the rats.