摘要目的:探讨高压氧(HBO)处理对胃癌SGC7901细胞氧分压及恶性生物学行为的影响。方法:胃癌细胞株SGC7901培养完成后，按照随机数字表法分为HBO组和对照组，对照组不予以任何处理，HBO组SGC7901细胞予以HBO处理。采用血气分析仪检测细胞培养基中的氧分压，倒置显微镜观察HBO处理前后SGC7901细胞形态的变化，Hoechst33258染色检测HBO处理后细胞的凋亡情况，Western blotting实验检测HBO处理后SGC7901细胞中缺氧诱导因子-1α(HIF-1α)蛋白的表达水平。结果:血气分析仪可以顺利且准确地检测SGC7901细胞培养基中的氧分压。HBO处理后SGC7901细胞培养基中氧分压较对照组明显提高( P<0.05)；且HBO处理结束后细胞培养基氧分压缓慢降低。HBO处理后，SGC7901细胞形态变圆，细胞间连接松散；与对照组比较未见明显凋亡( P>0.05)；HIF-1α蛋白表达水平明显降低( P<0.05)。 结论:HBO处理能明显增加胃癌SGC7901细胞培养基中的氧分压，有效改善SGC7901细胞的低氧状态，并下调HIF-1α蛋白的表达。

abstractsObjective:To study the effects of hyperbaric oxygen (HBO) treatment on partial pressure of oxygen (PaO 2) and malignant biological behavior of gastric cancer cell line SGC7901. Methods:After culture, the gastric cancer cell line SGC7901 was divided into HBO group and control group. The control group did not receive any treatment, while the SGC7901 in the HBO group was treated with HBO. The PaO 2 in cell culture medium was measured by blood gas analyzer. Morphological changes of SGC7901 cells before and after HBO treatment were observed by an inverted microscope. The cell apoptosis after HBO treatment was detected by Hoechst33258 staining. The expression level of HIF-1α protein in SGC7901 cells after HBO treatment was detected by Western blotting. Results:The blood gas analyzer can accurately detect the PaO 2 of cell culture medium, and the PaO 2 of cell culture medium in the HBO group was significantly higher than that in the control group ( P<0.05); moreover, the PaO 2 of cell culture medium in the HBO group decreased slowly at different time points. After HBO treatment, the morphology of SGC7901 cells became round and the intercellular connection was loose. The Hoechst33258 staining showed that, after HBO treatment, no significant cell apoptosis was observed in SGC7901 cells in the HBO group as compared with the control group ( P>0.05). Western blotting results showed that the expression level of HIF-1α in the HBO group was significantly lower than that in the control group ( P<0.05). Conclusion:HBO treatment can obviously increase the PaO 2 in the culture medium of gastric cancer cell line SGC7901, effectively relieve the hypoxia state of SGC7901 cells, and down-regulate the expression of HIF-1α protein.