摘要目的观察具有抗高血压作用新药1-（2，6-二甲基苯氧基）-2-（3，4-二甲氧基苯乙氨基）丙烷盐酸盐（DDPH）对低氧内皮细胞条件培养液(HECCM)诱导的大鼠肺血管周细胞(PC)增殖和免疫表型转化有无抑制作用，为开发具有降压和防止肺血管构型重建新药物提供实验依据。方法实验根据不同条件培养液分为4组：常氧内皮细胞条件培养液（NECCM）组，NECCM+DDPH组，HECCM组和HECCM+DDPH组。应用细胞培养、免疫细胞化学染色、图像分析及流式细胞术观察HECCM和DDPH对PC平滑肌肌动蛋白(α-SM-actin)和增殖细胞核抗原(PCNA)的表达及细胞周期的影响。结果① HECCM组PC α-SM-actin 呈强阳性表达，CD34和S-100呈阴性反应，而其它各组PC α-SM-actin、CD34及S-100均呈阳性表达；② HECCM组PC α-SM-actin和PCNA的表达量分别是NECCM组的1.32倍(F=11.09，P=0.000 1)和1.24倍(F=2.89，P=0.025 7)，是HECCM+DDPH组的1.30倍(F=3.65，P=0.007 0)和1.21倍(F=2.63，P=0.041 4)；③HECCM组PC的G0-G1期细胞百分率分别比NECCM组和HECCM+DDPH组低11.7％和9.1％，S期细胞百分率分别高5.6％和4.2％，G2-M期细胞百分率分别高6.1％和4.9％；④DDPH对 HECCM引起的PCs合成α-SM-actin和PCNA增高的抑制率分别为23.4％和17.1％，对G0-G1期细胞进入S期增多的抑制率为8.3％。结论 HECCM促进PCs增殖并向平滑肌细胞表型转化，DDPH对此过程有抑制作用。故DDPH具有直接抑制低氧性肺血管构型重建、缓解肺动脉高压的效应。

abstractsObjective　To study the effects of 1-(2，6-dimethylphenoxy)-2-(3，4-dimethoxyphenylethylamino) propane hydrochloride (DDPH) on proliferation and immunophenotypes of newborn rat pulmonary vascular pericytes (PCs) induced by hypoxic endothelial cell conditioned medium (HECCM) from porcine pulmonary arteries. Methods　The PCs were divided into 4 groups according to the endothelial cell conditioned medium (ECCM)：normoxic ECCM (NECCM) group，NECCM+DDPH group，HECCM group and HECCM+DDPH group.Cell culture，immunocytochemical staining，image analysis and flow cytometry were used to investigate the effects of HECCM and DDPH on the expression of α-smooth muscle actin (α-SM-actin) and proliferating cell nuclear antigen (PCNA) and cell cycle in PCs. Results　The α-SM-actin antigen in the PCs in HECCM group was more positively expressed than that in the other three groups，but CD34 antigen and S-100 antigen were negatively expressed.The expression of α-SM-actin antigen，CD34 and S-100 was positive in the groups of NECCM，NECCM+DDPH and HECCM+DDPH.The expression of α-SM-actin and PCNA in HECCM group was 1.32 times (F=11.09，P=0.000 1) and 1.24 times (F=2.89，P=0.025 7) that in NECCM group，1.30 times (F=3.65，P=0.007 0) and 1.21 times (F=2.63，P=0.041 4) that in HECCM+DDPH group，respectively；The percentage of the cells in the G0-G1 phase in the HECCM group was lower by 11.7% and 9.1%，in S phase higher by 5.6% and 4.2%，in G2-M phase higher by 6.1% and 4.9% than that in the groups of NECCM，HECCM+DDPH，respectively. The inhibitory rates of DDPH on the increased α-SM-actin and PCNA syntheses in PCs induced by HECCM were 23.4％ and 17.1％ respectively.The inhibitory rate on the increased PCs from G0-G1 phase to S phase was 8.3％. Conclusion　DDPH might directly inhibit PCs from proliferation and immunophenotypical transformation of smooth muscle-like cells induced by HECCM.