• 医学文献
  • 知识库
  • 评价分析
  • 全部
  • 中外期刊
  • 学位
  • 会议
  • 专利
  • 成果
  • 标准
  • 法规
  • 临床诊疗知识库
  • 中医药知识库
  • 机构
  • 作者
热搜词:
换一批
论文 期刊
取消
高级检索

检索历史 清除

医学文献>>
  • 全部
  • 中外期刊
  • 学位
  • 会议
  • 专利
  • 成果
  • 标准
  • 法规
知识库 >>
  • 临床诊疗知识库
  • 中医药知识库
评价分析 >>
  • 机构
  • 作者
热搜词:
换一批

重组腺相关病毒介导的RNA干扰抑制血管生长素表达对肺腺癌细胞成瘤能力的影响

Recombinant adeno-associated virus mediated RNA interference of angiogenin expression inhibits cell growth of human lung adenocarcinoma

摘要目的 通过腺相关病毒(AAV)介导的RNA干扰(RNAi)抑制肺腺癌细胞A549血管生长素(ANG)表达,观察其对癌细胞生长和成瘤能力的影响.方法 构建H1启动子驱动的表达针对ANG的小干扰RNA(siRNA)重组腺相关病毒(AAV-shANG),转染A549细胞,同时以正常A549细胞以及转染AAV-Null的A549细胞作为对照,观察重组腺相关病毒介导的RNAi抑制ANG表达对A549细胞生长、致瘤、肿瘤细胞增殖、凋亡及肿瘤微血管密度的影响.用t检验分析各组间差别,所有数据均用x±s表示.结果 体外实验结果表明重组腺相关病毒AAV-shANG成功构建,重组腺相关病毒转染A549细胞72 h后ANG蛋白表达水平明显低于A549细胞组及AAV-Null组;转基因A549细胞细胞周期分析结果提示,正常A549细胞、AAV-Null转染细胞和AAV-shANG转染细胞的增殖指数(PI)分别为0.32±0.29、0.35±0.38和0.31±0.43,差异不明显.体内实验结果表明,AAV-shANG转染细胞组胸腺缺陷小鼠的成瘤体积、瘤质最均明显低于两对照组.各组微血管密度分别为9.4±1.5、9.8±2.1和5.7±1.9,提示AAV-shANG转染细胞组与正常A549细胞和AAV-Null转染细胞组有明显差异.各组凋亡细胞的百分率分别为(7.7±3.1)%、(8.5±5.4)%和(17.1±8.6)%.AAV-shANG转染细胞组凋亡细胞的百分率明显高于正常A549细胞组和AAV-Null转染细胞组,各组细胞增殖核抗原(PCNA)阳性表达率分别为(84.8±9.7)%、(85.8±9.8)%、(70.4±10.1)%,AAV-shANG转染细胞组PCNA表达率低于两对照组.结论 AVV-siRNA表达能显著抑制肿瘤细胞ANG表达及肿瘤细胞增殖,促进肿瘤细胞凋亡,抑制肿瘤生长.

更多

abstractsObjective To study the inhibition of angiogenin (ANG) expression in human lung squamous cancer cell strain-A549 through adeno-associated virus (AAV)-mediated RNA-interference, and therefore to observe its effect on the growth of cancer cells and tumor formation. Methods Recombinant AAV expressing H1-promoter-induced small-interference-RNA(siRNA) targeting ANG (AAV-shANG) was constructed, and then transfected into A549 cells. A549 cells and cells transfected with AAV-Null were used as the control groups. The effects of the reduced expression of ANG by RNAi from AAV-shANG on the growth, formation, reproduction, apoptosis, and microvessel-density of the carcinoma were observed. Results In vitro experiment showed that AAV-shANG was constructed successfully, There was an significant decrease in the expression of ANG protein 72 h after transfection, compared with the normal A459 cells and AAV-Null cells (P<0.01). Cell cycle analysis showed that the proliferation index(PI) of normal A549 cells, AAV-Null cells and AAVshANG cells were 0.32±0.29, 0.35±0.38 and 0.31±0.43, respectively. There was no statistic difference in the PIs among the 3 groups (P>0.05). In vivo experiment using thymus-defect mice showed that, there was an remarkable reduction in the mass and volume of tumors in AAV-shANG transfected group, compared to the control groups. Microvessel-density was 9.4±1.5, 9.8±2.1 and 5.7±1.9, respectively in the 3 groups, a statistic difference among the AAV-shANG-transfected group, the normal A.549 group and the AAV-Null transfected group. The percentages of apoptotic cells in each group were (7.7±3.1) %, (8.5±5.4)%, (17.1±8.6) %, respectively, the experimental group being higher than those of the control groups. Positive rates of PCNA were (84.8±9.7) %, (85.8± 9.8)% ,and (70.4±10.1)%, respectively, the AAV-shANG transfected cancer cells showing a lower PCNA index than the control groups. Conclusion AAV-mediated expression of siRNA could reduce the erpression of ANG in cancer cells, significantly enough to inhibit cell proliferation, promote cell apoptosis and inhibit tumor growth.

More
广告
分类号 R73
栏目名称 论著
DOI 10.3760/cma.j.issn.1001-0939.2009.03.007
发布时间 2009-05-12
基金项目
国家自然科学基金 上海市科学技术委员会专项基金重点项目
  • 浏览416
  • 下载24
中华结核和呼吸杂志

中华结核和呼吸杂志

2009年32卷3期

188-192页

MEDLINEISTICPKUCSCD

加载中!

相似文献

  • 中文期刊
  • 外文期刊
  • 学位论文
  • 会议论文

加载中!

加载中!

加载中!

加载中!

扩展文献

特别提示:本网站仅提供医学学术资源服务,不销售任何药品和器械,有关药品和器械的销售信息,请查阅其他网站。

  • 客服热线:4000-115-888 转3 (周一至周五:8:00至17:00)

  • |
  • 客服邮箱:yiyao@wanfangdata.com.cn

  • 违法和不良信息举报电话:4000-115-888,举报邮箱:problem@wanfangdata.com.cn,举报专区

官方微信
万方医学小程序
new翻译 充值 订阅 收藏 移动端

官方微信

万方医学小程序

使用
帮助
Alternate Text
调查问卷