摘要目的 初步探讨COPD患者肺组织中β-连环蛋白的表达.方法 2011年4-9月华中科技大学同济医学院附属同济医院因肺部占位性病变行肺叶切除术患者36例,根据肺功能检查结果及吸烟史,分为吸烟+ COPD组、吸烟组和对照组,每组12例.尽量远离病变组织取外周肺组织,采用免疫荧光、Western blot和实时荧光定量逆转录-PCR技术,检测肺组织中β-连环蛋白的蛋白质及基因水平的表达.多组间比较采用单因素方差分析,两组间比较采用q检验,计数资料比较采用卡方检验,相关性检验采用Pearson相关分析方法.结果 β-连环蛋白主要位于上皮细胞的细胞膜,上皮细胞的细胞质和细胞核内也有阳性表达,吸烟+COPD组肺泡上皮细胞的细胞质和(或)细胞核中β-连环蛋白阳性表达细胞数为(1.2±0.6)/HP,肺组织中β-连环蛋白的蛋白和mRNA表达的吸光度值分别为0.26 ±0.11和0.35 ±0.13,均显著少于吸烟组和对照组[(5.0±2.5)/HP和(8.4±3.5)/HP,0.62±0.23和1.00 ±0.50,0.60 ±0.14和1.03±0.27],差异均有统计学意义(F值为12.809～38.776,均P＜0.05)；肺组织内β-连环蛋白的总蛋白和mRNA相对表达量与FEV1占预计值％及FEV1/FVC呈显著正相关(r值为0.402 ～0.558,P＜0.05).结论 吸烟并患COPD者的肺组织中β-连环蛋白表达显著低于吸烟未患COPD者,β-连环蛋白在肺组织中的表达量与肺功能呈正相关.

abstractsObjective To investigate the expression of β-catenin in pulmonary tissues of smokers with and without chronic obstructive pulmonary disease (COPD).Methods Pulmonary tissues were obtained from patients who had underwent pneumonectomy in Tongji Hospital.The subjects were assigned into non-smokers without COPD (control group),smokers without COPD (smoker group) and smokers with COPD (smoker + COPD group) based on their pulmonary functions and smoking history,with 12 subjects each group.The specimens were obtained as far from the tumor focus (＞ 5 cm) as possible.Immunofluorescence staining,Western blot and real-time quantitative reverse transcriptase polymerase chain reaction (RT-PCR) were used to investigate the expression and localization of 3-catenin in pulmonary tissues.Numerical data were expressed as the mean ± standard deviation,and were assessed for significance by one-way analysis of variance followed by a Student-Newman-Keuls test for multiple comparisons.The difference of enumeration data was detected by Chi-Square test.Relationship was estimated by Pearson correlation.Results Immunofluorescence analysis revealed that β-catenin mainly expressed in the cell membrane of epithelial cells.There was also a positive expression in the cytoplasm and the nuclei of the epithelial cells.The number of alveolar epithelial cells with β-catenin expressed in the cytoplasm and (or) nucleus was (1.2 ±0.6)/HP in smokers + COPD group.And the protein and mRNA expression of β-catenin in pulmonary tissues in smokers + COPD group were 0.26 ± 0.1 1 and 0.351 ±0.129,respectively,which were significantly less than those of the smoker group and the control group [(5.0 ±2.5)/HP and (8.4 ±3.5)/HP,0.62 ±0.23 and 1.00 ±0.50,0.60 ±0.14 and 1.03 ±0.27].The differences among the 3 groups were significant (F =12.809-38.776,P ＜ 0.05).Correlation analysis between β-catenin expression and pulmonary function suggested that the protein and mRNA expression of β-catenin positively related with FEV1％ pred (P ＜ 0.05) and FEV1/FVC (r =0.402-0.558,P ＜ 0.05).Conclusion β-catenin expression significantly was decreased in smokers with COPD,and β-catenin level in the lungs was positively correlated with pulmonary function.