摘要目的:探讨健康体检人群解偶联蛋白2（UCP2）及肥胖相关（FTO）基因多态性与内脏脂肪面积的关联性。方法:本研究为回顾性队列研究，连续收集2020年1月至2021年12月在武汉科技大学附属孝感医院健康管理部进行体检且腹部肚脐断面CT影像中的内脏脂肪面积≥100 cm 2的120例健康体检人群为超标组，以及同期在院体检且内脏脂肪面积<100 cm 2的120例健康体检人群为正常组。收集两组年龄、性别、身高、体重、体重指数、内脏脂肪面积等基本资料，同时进行基因型测定，采用logistic回归分析UCP2及FTO基因多态性与内脏脂肪面积的关联性。 结果:超标组体重、体重指数、总胆固醇、甘油三酯、低密度脂蛋白胆固醇（LDL-C）水平均显著高于正常组［（78.74±5.35）比（65.83±4.22）kg、（27.89±3.24）比（23.43±2.91）kg/m 2、（3.21±1.51）比（2.32±1.47）mmol/L、（5.29±1.34）比（4.86±1.16）mmol/L、（2.73±0.89）比（2.51±0.82）mmol/L］，高密度脂蛋白胆固醇（HDL-C）水平显著低于正常组［（1.02±0.31）比（1.23±0.43）mmol/L］（均 P<0.05）；两组年龄、性别、身高、空腹血糖、糖化血红蛋白差异均无统计学意义（均 P>0.05）。超标组UCP2-866A/G基因位点中的AG/GG等位基因、FTO rs9939609基因位点中的TA、AA、A等位基因的表达比例均显著高于正常组（82.50%比69.17%、38.33%比19.17%、7.50%比4.17%、23.75%比15.00%）（均 P<0.05）。UCP2基因（ OR=2.303，95% CI：1.456~3.642）及FTO基因（ OR=2.782，95% CI：1.149~6.733）表达均与内脏脂肪面积升高呈正相关（均 P<0.05）。 结论:健康体检人群UCP2-866A/G或FTO rs9939609基因位点呈现多态性表达时，内脏脂肪面积升高的风险会显著增加。

abstractsObjective:To investigate the correlation between uncoupling protein 2 (UCP2) and fat mass and obesity associated (FTO) gene polymorphisms and visceral fat area in a population undergoing health examinations.Methods:In this retrospective cohort study, 120 people who underwent physical examination in the Health Management Department of Xiaogan Hospital Affiliated to Wuhan University of Science and Technology from January 2020 to December 2021 with a visceral fat area ≥100 cm 2 under the abdominal navel section CT images were included in the superstandard group. In addition, 120 people who received health checkup in the meantime with a visceral fat area <100 cm 2 were included in the normal group. Basic information including age, gender, height, weight, body mass index (BMI), and visceral fat area was collected for both groups. Genotyping was performed, and logistic regression analysis was used to determine the association between UCP2 and FTO gene polymorphisms and visceral fat area. Results:The weight, body mass index, total cholesterol, triglyceride and low density lipoprotein cholesterol (LDL-C) in the superstandard group were all significantly higher than those in the normal group [(78.74±5.35) vs (65.83±4.22) kg, (27.89±3.24) vs (23.43±2.91) kg/m 2, (3.21±1.51) vs (2.32±1.47) mmol/L, (5.29±1.34) vs (4.86±1.16) mmol/L, (2.73±0.89) vs (2.51±0.82) mmol/L], the level of high density lipoprotein cholesterol (HDL-C) was significantly lower than that in normal group [(1.02±0.31) vs (1.23±0.43) mmol/L] (all P<0.05); there were no significant differences in age, gender, height, fasting blood glucose and glycated hemoglobin between the two groups (all P>0.05). The expression ratio of AG/GG allele in UCP2-866A/G and TA, AA and A allele in FTO rs9939609 gene locus in superstandard group were all significantly higher than those in normal group (82.50% vs 69.17%, 38.33% vs 19.17%, 7.50% vs 4.17%, 23.75% vs 15.00%) (all P<0.05). The expression of UCP2 gene ( OR=2.303, 95% CI: 1.456-3.642) and FTO gene ( OR=2.782, 95% CI: 1.149-6.733) were positively correlated with increased visceral fat area (both P<0.05). Conclusion:The presence of UCP2-866A/G or FTO rs9939609 gene polymorphisms in individuals undergoing health examinations significantly increases the risk of increased visceral fat area.