摘要目的 探讨局部应用唑来膦酸(zoledronic acid)对拔牙创愈合过程中Ⅰ型胶原和血管内皮生长因子(vascular endothelial growth factor,VEGF)的影响.方法 54只雄性Wistar大鼠按照随机数字法分为3组:唑来膦酸治疗组、明胶海绵组、空白对照组,每组18只,均拔除同一侧下颌第一磨牙.唑来膦酸治疗组于拔牙创内植入唑来膦酸-明胶海绵复合体;明胶海绵组植入明胶海绵;空白对照组不作处理.分别于术后1、2、4周取标本,应用免疫组化SP法观察Ⅰ型胶原和VEGF的表达,并对染色灰度值进行统计学分析.结果 术后1、2周,唑来膦酸治疗组Ⅰ型胶原灰度值(60.00±1.81、63.47±3.02)均显著低于明胶海绵组(68.58±2.90、71.15±5.57)和空白对照组(69.16±9.63、72.50±4.10)(P<0.05);唑来膦酸治疗组和明胶海绵组4周Ⅰ型胶原灰度值高于1、2周(P<0.05).术后1周,唑来膦酸治疗组VEGF灰度值(69.93±2.74)较明胶海绵组(60.86±4.79)及空白对照组(61.52±2.28)高(P<0.05),唑来膦酸治疗组2周较1、4周低,差异有统计学意义(P<0.05).结论 唑来膦酸局部用药可增加Ⅰ型胶原的表达,但早期抑制VEGF的表达.

abstractsObjective To investigate the effect of local application of zoledronic acid(ZA)on the expression of typeⅠcollagen and vascular endothelial growth factor(VEGF). Methods Fifty-four male Wistar rats were divided into ZA group, gelatin sponge group, and blank control group after one-side tooth extraction. The expression of typeⅠcollagen and VEGF were detected with SP immunohistochemistry method 1, 2 and 4 weeks after operation. Results The gray value of typeⅠcollagen in ZA group (60.00±1.81,63.47±3.02)was lower than those in gelatin sponge group(68.58±2.90,71.15±5.57) and blank control group(69.16±9.63,72.50±4.10,P<0.05)in the 1 and 2 week.In the ZA and gelatin sponge groups, the gray values of typeⅠcollagen were higher in the 4th week than in the 1st and 2nd week(P<0.05). The expression of VEGF was higher in ZA group (69.93±2.74)than in gelatin sponge group(60.86±4.79)and blank control group (61.52±2.28)in the 1st week(P<0.05). There was significant difference in VEGF expression between the 2nd week and 1st and 4th week(P<0.05). Conclusions Local application of ZA could enhance the expression of typeⅠcollagen but inhibit the expression of VEGF in the early stage.