摘要目的 观察白色念珠菌对烟曲霉菌生长的抑制作用,筛选适用于临床的烟曲霉菌选择性培养基.方法 将不同浓度的白色念珠菌和烟曲霉菌分别在沙保罗葡萄糖琼脂(SDA)液体培养基和平皿上单独和共培养,观察并记录烟曲霉菌生长与否、是否产色素,并通过菌落直径来判断其生长受抑制的程度.同时,将两者在含不同浓度氟康唑的SDA平皿上25℃恒温培养,观察和记录两者生长与否以及烟曲霉菌菌落的直径.结果 ①SDA用于培养白色念珠菌和烟曲霉菌的敏感性为100CFU/ml.②在液体SDA中,106CFU/ml的白色念珠菌能完全抑制103CFU/ml和106CFU/ml烟曲霉菌的生长.③在不同比例的烟曲霉菌和白色念珠菌的混合菌悬液中,烟曲霉菌的生长均受到抑制,其受抑制的程度与白色念珠菌的浓度呈正相关.④在含5 mg/L氟康唑的SDA上白色念珠菌不生长,而烟曲霉菌菌落的直径与对照组相比无统计学差异.结论 体外试验证实,白色念珠菌对烟曲霉菌生长有抑制作用,随着白色念珠菌浓度的升高,抑制效应更明显,含5 mg/L氟康唑的SDA可作为烟曲霉菌的选择性培养基.

abstractsObjective To investigate the growth inhibition of Aspergillns fumigatus by Candida albicans in vitro and to develop the selective medium for clinical isolation of Aspergillus fumigatus.Methods Aspergillus fumigatus and Candida albicans were single or co-cultured in sabouraud dextrose agar(SDA) medium and SDA broth in dark at 25 ℃,and fungal growth,pigmentation,as well as colony diameter were documented.Results ①The sensitivity of culture of Aspergillus fumigatus and Candida albicans on SDA plate was 100CFU/ml.②The growth of 106CFU/ml and 103CFU/ml Aspergills fumigatus was completely inhibited by 106CFU/ml Candida albicans.③Growth inhibition of Aspergillus fumigatus was correlated with the concentration of Candida albicans.④SDA containing 1 mg/L fluconazole inhibited growth of Candida albicans,and no Candida albicans was detected on SDA containing 5 mg/L and 25 mg/L fluconazole.Growth of Aspergillus fumigatus was partially inhibited on SDA containing 25 mg/L fluconazole.Conclusions Candida albicans can inhibit the growth of Aspergillus fumigatus in vitro.SDA containing 5 mg/L fluconazole can be used as the selective medium for the isolation of Aspergillus fumigatus.