摘要目的 研究ICR乳鼠对实验感染人轮状病毒(human rotavirus,HRV)Wa株的敏感性.方法 选用60只ICR乳鼠,共6窝,随母鼠按窝随机均分为Ⅰ、Ⅱ2个大组,6个小组.第Ⅰ组包括A、B、C 3个小组,第Ⅱ组包括D、E、F 3个小组,其中B、C、E、F组为实验组,A、D组为对照组.B、C组分别用3×10~5PFU、3×10~6PFU HRV Wa株悬液感染4日龄乳鼠,E、F组分别用3×10~5PFU、3×10~6PFU HRV液感染10日龄乳鼠,观察各组乳鼠的临床反应、排毒时间、小肠组织病理学及超微结构改变.采用单因素方差分析(one-way ANOVA)比较各组数据差异.结果 第1组乳鼠腹泻天数、排毒时间、空肠绒毛高度、感染病毒后第4和第7天的体质量比较差异有统计学意义(F值分别204.38、86.60、7.18、18.41和10.08,P值均<0.01);第Ⅱ组乳鼠均无腹泻,空肠绒毛高度及感染病毒后第4和第7天的体质量比较差异无统计学意义(F值分别0.16、0.13和1.03,P值均>0.05),但E和F组的排毒时间明显长于D组(F=8.34,P<0.01).结论 4日龄ICR乳鼠感染3×10~6PFU HRV Wa株可建立ICR乳鼠的HRV腹泻模型.

abstractsObjective To establish an animal model of human rotavirus(HRV)-induced diarrhea. Methods Sixty ICR suckling mice were randomly divided into two groups, and each was further divided into 3 subgroups(A, B, C for group Ⅰ, and D, E, F for group Ⅱ);group B, C, E and F were assigned as experimental groups. while group A and D were controls. Mice in group B and C were inoculated with3 × 10~5 PFU and 3×10~6 PFU HRV Wa strain fluid respectively when they were 4-day old. Mice in group E and F were inoculated with 3×10~5 PFU and 3×10~6 PFU HRV Wa strain fluid respectively when they were10-day old. The symptoms, fecal viral excretion, intestinal histopathologies and ultrastructures of animals were observed. One-way ANOVA was used to compare the differences among the groups. Results There were significant differences in the duration of diarrhea and viral excretion, jejunal villous height, the weight at d4 and d7 after inoculation among three subgroups in group Ⅰ(F=204.38, 86.60, 7.18, 18.41 and10.08, P<0.01). Diarrhea was not observed in group Ⅱ, and the differences in jejunal villous height and the weight at d4 and d7 after inoculation among three subgroups were not significant(F=0.16, 0.13 and 1.03, P>0.05). Compared with the group D, the duration of viral excretion was longer in group E and F(F=8. 34, P<0.01). Conclusion Animal model of HRV diarrhea can be established in 4-day-old ICR suckling mice infected with 3×10~6PFU HRV.