分离自烧伤患者的鲍曼不动杆菌可移动遗传元件研究
Mobile genetic elements carried by Acinetobacter baumannii strains isolated from burn patients
摘要目的 研究烧伤患者鲍曼不动杆菌分离株可移动遗传元件的携带状况.方法 采用聚合酶链反应(PCR)方法对分离自烧伤患者的20株鲍曼不动杆菌的整合子、转座子、接合性质粒和插入序列等编码基因进行检测.结果 20株鲍曼不动杆菌检测到intⅠ基因19株(95%),tnpU基因20株(100%),ISaba1基因20株(100%),其余基因均为阴性.结论 分离自烧伤患者的鲍曼不动杆菌携带多种可移动遗传元件,临床上应引起足够重视.
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abstractsObjective To investigate the prevalence of mobile genetic elements in Acinetobacter baumannii strains isolated from burn patients. Methods Polymerase chain reaction (PCR) was used to detect the genes encoding the integron, transposon, conjugative plasmid and insertion sequence in 20 strains of Acinetobacter baumannii isolated from burn patients. Results tnpU and ISaba1 genes were detected in all 20 strains, and int Ⅰ gene was detected in 19 strains (95.0%). Other genes were all negative. Conclusion Mobile genetic elements carrying multi-drug resistant genes are found in Acinctobacter baumannii strains isolated from bum patients.
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