摘要目的 观察Smad2/3、Smad4、磷酸化Smad3( P-Smad3)蛋白在人肝纤维化组织中的表达,探讨Smad蛋白及其介导的生物学信号在肝纤维化发生中的作用机制.方法 以肝组织活检病理诊断区分131例慢性HBV感染者纤维化程度,免疫组化法检测肝组织中Smad2/3、Smad4和P-Smad3蛋白的表达并进行定量分析.结果Smad2/3、Smad4、P-Smad3蛋白主要见于纤维间隔、汇管区和中央静脉周围成纤维细胞、肝窦及部分肝细胞质内均可见表达.肝组织Smad2/3、Smad4、P-Smad3蛋白与肝纤维化程度有非常显著性正相关(r分别是0.81、0.58和0.68,P=0.000).三种蛋白间也具有非常显著性正相关(r分别是0.75、0.87和0.84,P=0.000).结论 肝组织Smad2/3、Smad4、P-Smad3蛋白表达与慢性HBV感染肝纤维化程度相关,Smad2/3、P-Smad3、Smad4信号的增强可能促讲了肝纤维化的讲展.

abstractsObjective To evaluate the association of Smad2/3,Smad4 and P-Smad3 protein expressions in tissue with liver fibrosis in patients with chronic HBV infections.Methods Liver biopsy was performed to determine the liver fibrosis grades in 131 cases of chronic HBV infections.Immunohistochemistry and semiquantitative analysis were applied to detect the expression of Smad2/3,Smad4 and P-Smad3 proteins in liver tissues.Results Smad2/3,Smad4 and P-Smad3 were detected mainly in fibrous septum,portal areas,myofibroblasts,sinus and cytoplasm in the liver tissue.The expression of Smad2/3,Smad4 and P-Smad3 was increased with the development of fibrosis ( r =0.81,0.58and 0.68,P =0.000),and a strong positive correlation was observed among three proteins (r =0.75,0.87and 0.84,P =0.000).Conclusion The expression of Smad2/3,Smad4 and P-Smad3 is correlated with liver fibrosis in chronic HBV infection,which suggests that the up-regulation of Smad proteins may be involved in the progression of liver fibrosis.