摘要目的 探讨PKA的锚定蛋白A-kinase anchor protein 95 (AKAP95)、细胞周期蛋白CyclinE1蛋白和CyclinD1蛋白在肺癌组织中表达的关联性.方法 应用免疫组化方法检测51例肺癌组织中AKAP95、CyclinE1和CyclinD1蛋白的表达.结果 1.CyclinE1蛋白在肺癌中的表达水平明显高于癌旁组织,阳性率分别为75.56％和20.00％,差异有统计学意义(P＜0.01);其表达与组织学分型、淋巴结是否发生转移和分化程度均无关,差异无统计学意义(P＞0.05);2.CyclinD1蛋白在肺癌组织表达的阳性率为69.39％,高于癌旁组织的表达阳性率为(14.29％),其表达与组织学分型有关,差异有统计学意义(P＜0.05),3.AKAP95和CyclinE1蛋白、AKAP95和CyclinD1蛋白在肺癌组织中均呈关联性表达,差异有统计学意义(P＜0.01).结论 1.CyclinE1和CyclinD1蛋白在肺癌组织中高表达,提示在肺癌的发生发展中发挥作用;2.CyclinE1蛋白表达与肺癌的分化程度、组织学分型及淋巴结转移均无关;CyclinD1蛋白表达与组织学分型有关;3.AKAP95和CyclinE1蛋白、AKAP95和CyclinD1蛋白在肺癌组织中均呈关联性表达.

abstractsObjective To investigate the correlation between expression of A-kinase anchoring protein 95 (AKAP95) and protein expression of cyclin E1 and cyclin D1 in lung cancer tissue.Methods Fifty-one cases of lung cancer were included in the study.The protein expression of AKAP95,cyclin E1,and cyclin D1 were measured by immunohistochemistry.Results The protein expression of cyclin E1 in lung cancer tissues was significantly higher than that in paracancerous tissues (positive rate:75.56％ vs 20％,P＜0.01); its expression showed no relationship with histopathological type,lymph node metastasis,and cellular differentiation (P＞0.05).The protein expression of cyclin D1 in lung cancer tissues was higher than that in paracancerous tissues (positive rate:69.39％ vs 14.29％); its expression showed a significant relationship with histopathological type (P＜0.05).The expression of AKAP95 was correlated with the protein expression of cyclin E1 and cyclin D1 in lung cancer tissues (P＜0.01).Conclusion Cyclin E1 and cyclin D1 are highly expressed in lung cancer tissue,suggesting that they play an important role in the development and progression of lung cancer.The protein expression of cyclin E1 has no relationship with cellular differentiation,lymph node metastasis,and histopathological type of lung cancer,and the protein expression of cyclin D1 has a significant relationship with histopathological type.The expression of AKAP95 is correlated with the protein expression of cyclin E1 and cyclin D1 in lung cancer tissue.