摘要目的 观察HSV-TK/ACV系统对肾癌GRC-1细胞的杀伤作用及旁观者效应。方法 将含不同浓度的肾癌GRC-1/TK细胞与肾癌GRC-1细胞混合培养，分为4组，分别含GRC-1/TK细胞0％、25％、50％及100％，分别用无环鸟苷60μg/ml进行处理，观察细胞形态改变、测定活细胞数并行流式细胞术、电子显微镜检查。结果 实验组与对照组GRC-1细胞均无明显变化；含25％以上GRC-1/TK细胞可观察到明显的杀伤作用，并有凋亡峰及凋亡小体形成。结论 HSV-TK/ACV系统对肾癌GRC-1细胞具有明显的杀伤作用及旁观者效应，机制可能与细胞凋亡有关。

abstractsObjective To study bystander effect and cell death by HSV-TK andACV therapy on renal carcinoma cell lines GRC-1.Methods Different mix culture cells, which contained GRC-1/TK cells 0％、25％、50％ and 100%,were treated with ACV 60 μg/ml, the morphology and quantity of tumor cells being studied and checked with FCM and electron microscope.Results After exposure to ACV, GRC-1/TK cells, even only 25%, underwent cell death and bystander effect, but there was no obvious change in the morphology and quantity of GRC-1 cells exposed to ACV.Conclusions There were bystander effect and cell death on HSV-TK and ACV therapy in renal carcinoma cell lines GRC-1. The mechanism of bystander effect may have deat with apoptosis.