摘要目的 评价不同浓度不同时间七氟醚麻醉对小鼠认知功能的影响.方法 成年雄性C57BL/6小鼠50只,体重20～ 24 g,10周龄,采用随机数字表法,分为5组(n=10):正常对照组(C组)、吸入1.3％七氟醚0.5 h组(S1组)、吸入1.3％七氟醚2.0 h组(S2组)、吸入2.5％七氟醚2.0 h组(S3组)和吸入2.5％七氟醚4.0 h组(S4组).麻醉后2周进行Morris水迷宫实验,记录逃避潜伏期和穿越平台次数,水迷宫实验结束后处死,取海马组织,检测含2B亚基的NMDA受体和活化的caspase-3表达水平.结果 与C组比较,S1组、S2组和S3组逃避潜伏期缩短,穿越平台次数增加,S4组逃避潜伏期延长,S1组、S2组、S3组和S4组海马组织含2B亚基的NMDA受体表达上调,S3组和S4组海马组织活化的caspase-3表达上调(P＜0.05或0.01).结论 七氟醚麻醉对小鼠认知功能影响不同的原因与浓度和时间有关.

abstractsObjective To evaluate the effects of different concentrations and different duration of sevoflurane anesthesia on cognitive function in mice.Methods Fifty adult male C57BL/6 mice,weighing 20-24 g,aged 10 weeks,were randomly divided into 5 groups (n =10 each) using a random number table:control group (group C),1.3％ sevoflurane inhaled for0.5 h group (group S1),1.3％ sevoflurane inhaled for 2.0 h group (group S2),2.5％ sevoflurane inhaled for 2.0 h group (group S3),and 2.5％ sevoflurane inhaled for 4.0 h group (group S4).At 2 weeks after anesthesia,Morris water maze test was performed,and the escape latency and frequency of crossing the original platform were recorded.After the end of the test,the mice were sacrificed,and brains were removed to detect the expression of 2B subunit-containing N-methyl-D-aspartate receptors (NR2B) and activated caspase-3 in the hippocampus.Results Compared with group C,the escape latency was significantly shortened,and the frequency of crossing the original platform was increased in S1-3 groups,and the escape latency was prolonged,and no significant change was found in the frequency of crossing the original platform in group S4.Compared with group C,the expression of NR2B in hippocampal tissues was up-regulated in S1-4 groups,and the expression of activated caspase-3 in hippocampal tissues was up-regulated in S3 and S4 groups.Conclusion The reason for different effects of sevoflurane anesthesia on cognitive function is related to the concentration and duration in mice.